Dopaminergic regulation of prolactin and growth hormone synthesis in rat pituitary tumor cells

Cecilia Y. Cheung; Shlomo Melmed; Glenn D. Braunstein

doi:10.1016/0006-8993(83)90808-9

Dopaminergic regulation of prolactin and growth hormone synthesis in rat pituitary tumor cells

Cecilia Y. Cheung, Shlomo Melmed, Glenn D. Braunstein

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The dopaminergic regulation of prolactin (PRL) and growth hormone (GH) synthesis were studied in monolayer cultures of rat pituitary tumor (GH₃) cells. PRL synthesis could not be inhibited by dopamine at concentrations ranging from 10^-9 to 10^-5 M. Bromocriptine inhibited PRL synthesis only at the very high concentration of 10^-5 M, and this effect was not reversible by metoclopramide. GH synthesis was inhibited by 10^-6 and 10^-5 M dopamine but not by bromocriptine, and the dopamine effect was blocked by metoclopramide. These results suggest the existence of a defective dopaminergic regulation of PRL and GH synthesis in GH₃ cells.

Original language	English (US)
Pages (from-to)	165-168
Number of pages	4
Journal	Brain research
Volume	270
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)90808-9
State	Published - Jun 27 1983
Externally published	Yes

Keywords

dopaminergic regulation
growth hormone
pituitary tumor
prolactin
rat

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(83)90808-9

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title = "Dopaminergic regulation of prolactin and growth hormone synthesis in rat pituitary tumor cells",

abstract = "The dopaminergic regulation of prolactin (PRL) and growth hormone (GH) synthesis were studied in monolayer cultures of rat pituitary tumor (GH3) cells. PRL synthesis could not be inhibited by dopamine at concentrations ranging from 10-9 to 10-5 M. Bromocriptine inhibited PRL synthesis only at the very high concentration of 10-5 M, and this effect was not reversible by metoclopramide. GH synthesis was inhibited by 10-6 and 10-5 M dopamine but not by bromocriptine, and the dopamine effect was blocked by metoclopramide. These results suggest the existence of a defective dopaminergic regulation of PRL and GH synthesis in GH3 cells.",

keywords = "dopaminergic regulation, growth hormone, pituitary tumor, prolactin, rat",

author = "Cheung, {Cecilia Y.} and Shlomo Melmed and Braunstein, {Glenn D.}",

note = "Funding Information: The authorsa re gratefult o Judith Seliktar and Marilyn Leungf or excellentte chnicaal ssistancaen d to Helene Zaudererfo r secretariael xpertiseS. up-ported by NIADKD Clinical InvestigatoAr ward AM 00906( S.M.); BioScienceL aboratorieEs ndo-crine ResearchF ellowshipa nd NIH Grant BRSG RR-05468.",

year = "1983",

month = jun,

day = "27",

doi = "10.1016/0006-8993(83)90808-9",

language = "English (US)",

volume = "270",

pages = "165--168",

journal = "Brain Research",

issn = "0006-8993",

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number = "1",

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T1 - Dopaminergic regulation of prolactin and growth hormone synthesis in rat pituitary tumor cells

AU - Cheung, Cecilia Y.

AU - Melmed, Shlomo

AU - Braunstein, Glenn D.

N1 - Funding Information: The authorsa re gratefult o Judith Seliktar and Marilyn Leungf or excellentte chnicaal ssistancaen d to Helene Zaudererfo r secretariael xpertiseS. up-ported by NIADKD Clinical InvestigatoAr ward AM 00906( S.M.); BioScienceL aboratorieEs ndo-crine ResearchF ellowshipa nd NIH Grant BRSG RR-05468.

PY - 1983/6/27

Y1 - 1983/6/27

N2 - The dopaminergic regulation of prolactin (PRL) and growth hormone (GH) synthesis were studied in monolayer cultures of rat pituitary tumor (GH3) cells. PRL synthesis could not be inhibited by dopamine at concentrations ranging from 10-9 to 10-5 M. Bromocriptine inhibited PRL synthesis only at the very high concentration of 10-5 M, and this effect was not reversible by metoclopramide. GH synthesis was inhibited by 10-6 and 10-5 M dopamine but not by bromocriptine, and the dopamine effect was blocked by metoclopramide. These results suggest the existence of a defective dopaminergic regulation of PRL and GH synthesis in GH3 cells.

AB - The dopaminergic regulation of prolactin (PRL) and growth hormone (GH) synthesis were studied in monolayer cultures of rat pituitary tumor (GH3) cells. PRL synthesis could not be inhibited by dopamine at concentrations ranging from 10-9 to 10-5 M. Bromocriptine inhibited PRL synthesis only at the very high concentration of 10-5 M, and this effect was not reversible by metoclopramide. GH synthesis was inhibited by 10-6 and 10-5 M dopamine but not by bromocriptine, and the dopamine effect was blocked by metoclopramide. These results suggest the existence of a defective dopaminergic regulation of PRL and GH synthesis in GH3 cells.

KW - dopaminergic regulation

KW - growth hormone

KW - pituitary tumor

KW - prolactin

KW - rat

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U2 - 10.1016/0006-8993(83)90808-9

DO - 10.1016/0006-8993(83)90808-9

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AN - SCOPUS:0020620899

SN - 0006-8993

VL - 270

SP - 165

EP - 168

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Dopaminergic regulation of prolactin and growth hormone synthesis in rat pituitary tumor cells

Abstract

Keywords

ASJC Scopus subject areas

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