Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors

Marina E. Wolf, R. H. Roth

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The ability of dopamine (DA) agonists and antagonists to modulate the K+-evoked overflow of radioactivity from superfused slices of prefrontal cortex of the rat, preincubated with [3H]DA in the presence of 1 μM desipramine, was examined. Apomorphine and the putative autoreceptor-selective DA agonist EMD 23 448 inhibited the K+-evoked overflow of radioactivity, while the DA antagonist sulpiride enhanced the evoked overflow in a dose-dependent and stereoselective manner. The latter effect was partially reversed by EMD 23 448. More than 95% of the radioactivity retained by the slices chromatographed with DA, while deaminated metabolites represented the majority of both the basal efflux (84% metabolites, 4-5% DA) and evoked overflow (84% metabolites, 14% DA) of radioactivity. These findings indicate that mesoprefrontal DA neurons possess release-modulating nerve terminal autoreceptors. Previous studies have shown that these neurons lack synthesis-modulating autoreceptors. Thus, autoreceptors on prefrontal DA terminals appear to be coupled to regulation of the release but not the synthesis of DA.

Original languageEnglish (US)
Pages (from-to)1053-1059
Number of pages7
JournalNeuropharmacology
Volume26
Issue number8
DOIs
StatePublished - Aug 1987

Keywords

  • brain slices
  • dopamine autoreceptors
  • dopamine release
  • prefrontal cortex

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors'. Together they form a unique fingerprint.

  • Cite this