Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors

Marina Wolf, R. H. Roth

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The ability of dopamine (DA) agonists and antagonists to modulate the K+-evoked overflow of radioactivity from superfused slices of prefrontal cortex of the rat, preincubated with [3H]DA in the presence of 1 μM desipramine, was examined. Apomorphine and the putative autoreceptor-selective DA agonist EMD 23 448 inhibited the K+-evoked overflow of radioactivity, while the DA antagonist sulpiride enhanced the evoked overflow in a dose-dependent and stereoselective manner. The latter effect was partially reversed by EMD 23 448. More than 95% of the radioactivity retained by the slices chromatographed with DA, while deaminated metabolites represented the majority of both the basal efflux (84% metabolites, 4-5% DA) and evoked overflow (84% metabolites, 14% DA) of radioactivity. These findings indicate that mesoprefrontal DA neurons possess release-modulating nerve terminal autoreceptors. Previous studies have shown that these neurons lack synthesis-modulating autoreceptors. Thus, autoreceptors on prefrontal DA terminals appear to be coupled to regulation of the release but not the synthesis of DA.

Original languageEnglish (US)
Pages (from-to)1053-1059
Number of pages7
JournalNeuropharmacology
Volume26
Issue number8
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Autoreceptors
Dopaminergic Neurons
Prefrontal Cortex
Dopamine
Radioactivity
Dopamine Antagonists
Dopamine Agonists
Sulpiride
Desipramine
Apomorphine
Neurons

Keywords

  • brain slices
  • dopamine autoreceptors
  • dopamine release
  • prefrontal cortex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors. / Wolf, Marina; Roth, R. H.

In: Neuropharmacology, Vol. 26, No. 8, 01.01.1987, p. 1053-1059.

Research output: Contribution to journalArticle

@article{b88d224520b24e1b8bb293bfc71b88dd,
title = "Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors",
abstract = "The ability of dopamine (DA) agonists and antagonists to modulate the K+-evoked overflow of radioactivity from superfused slices of prefrontal cortex of the rat, preincubated with [3H]DA in the presence of 1 μM desipramine, was examined. Apomorphine and the putative autoreceptor-selective DA agonist EMD 23 448 inhibited the K+-evoked overflow of radioactivity, while the DA antagonist sulpiride enhanced the evoked overflow in a dose-dependent and stereoselective manner. The latter effect was partially reversed by EMD 23 448. More than 95{\%} of the radioactivity retained by the slices chromatographed with DA, while deaminated metabolites represented the majority of both the basal efflux (84{\%} metabolites, 4-5{\%} DA) and evoked overflow (84{\%} metabolites, 14{\%} DA) of radioactivity. These findings indicate that mesoprefrontal DA neurons possess release-modulating nerve terminal autoreceptors. Previous studies have shown that these neurons lack synthesis-modulating autoreceptors. Thus, autoreceptors on prefrontal DA terminals appear to be coupled to regulation of the release but not the synthesis of DA.",
keywords = "brain slices, dopamine autoreceptors, dopamine release, prefrontal cortex",
author = "Marina Wolf and Roth, {R. H.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1016/0028-3908(87)90248-6",
language = "English (US)",
volume = "26",
pages = "1053--1059",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - Dopamine neurons projecting to the medial prefrontal cortex possess release-modulating autoreceptors

AU - Wolf, Marina

AU - Roth, R. H.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The ability of dopamine (DA) agonists and antagonists to modulate the K+-evoked overflow of radioactivity from superfused slices of prefrontal cortex of the rat, preincubated with [3H]DA in the presence of 1 μM desipramine, was examined. Apomorphine and the putative autoreceptor-selective DA agonist EMD 23 448 inhibited the K+-evoked overflow of radioactivity, while the DA antagonist sulpiride enhanced the evoked overflow in a dose-dependent and stereoselective manner. The latter effect was partially reversed by EMD 23 448. More than 95% of the radioactivity retained by the slices chromatographed with DA, while deaminated metabolites represented the majority of both the basal efflux (84% metabolites, 4-5% DA) and evoked overflow (84% metabolites, 14% DA) of radioactivity. These findings indicate that mesoprefrontal DA neurons possess release-modulating nerve terminal autoreceptors. Previous studies have shown that these neurons lack synthesis-modulating autoreceptors. Thus, autoreceptors on prefrontal DA terminals appear to be coupled to regulation of the release but not the synthesis of DA.

AB - The ability of dopamine (DA) agonists and antagonists to modulate the K+-evoked overflow of radioactivity from superfused slices of prefrontal cortex of the rat, preincubated with [3H]DA in the presence of 1 μM desipramine, was examined. Apomorphine and the putative autoreceptor-selective DA agonist EMD 23 448 inhibited the K+-evoked overflow of radioactivity, while the DA antagonist sulpiride enhanced the evoked overflow in a dose-dependent and stereoselective manner. The latter effect was partially reversed by EMD 23 448. More than 95% of the radioactivity retained by the slices chromatographed with DA, while deaminated metabolites represented the majority of both the basal efflux (84% metabolites, 4-5% DA) and evoked overflow (84% metabolites, 14% DA) of radioactivity. These findings indicate that mesoprefrontal DA neurons possess release-modulating nerve terminal autoreceptors. Previous studies have shown that these neurons lack synthesis-modulating autoreceptors. Thus, autoreceptors on prefrontal DA terminals appear to be coupled to regulation of the release but not the synthesis of DA.

KW - brain slices

KW - dopamine autoreceptors

KW - dopamine release

KW - prefrontal cortex

UR - http://www.scopus.com/inward/record.url?scp=0023251520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023251520&partnerID=8YFLogxK

U2 - 10.1016/0028-3908(87)90248-6

DO - 10.1016/0028-3908(87)90248-6

M3 - Article

C2 - 3658117

AN - SCOPUS:0023251520

VL - 26

SP - 1053

EP - 1059

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 8

ER -