Dopamine modulates the activity of sensory hair cells

Cecilia Toro, Josef G. Trapani, Itallia Pacentine, Reo Maeda, Lavinia Sheets, Weike Mo, Teresa Nicolson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The senses of hearing and balance are subject to modulation by efferent signaling, including the release of dopamine (DA). How DA influences the activity of the auditory and vestibular systems and its site of action are not well understood. Here we show that dopami-nergic efferent fibers innervate the acousticolateralis epithelium of the zebrafish during development but do not directly form synapses with hair cells. However, a member of the D1-like receptor family, D1b, tightly localizes to ribbon synapses in inner ear and lateral-line hair cells. To assess modulation of hair-cell activity, we reversibly activated or inhibited D1-like receptors (D1Rs) in lateral-line hair cells. In extracellular recordings from hair cells, we observed that D1R agonist SKF-38393 increased microphonic potentials, whereas D1R antagonist SCH-23390 decreased microphonic potentials. Using ratiometric calcium imaging, we found that increased D1R activity resulted in larger calcium transients in hair cells. The increase of intracellular calcium requires Cav1.3a channels, as a Cav1 calcium channel antagonist, isradipine, blocked the increase in calcium transients elicited by the agonist SKF-38393. Collectively, our results suggest that DA is released in a paracrine fashion and acts at ribbon synapses, likely enhancing the activity of presynaptic Cav1.3a channels and thereby increasing neurotransmission.

Original languageEnglish (US)
Pages (from-to)16494-16503
Number of pages10
JournalJournal of Neuroscience
Volume35
Issue number50
DOIs
StatePublished - Dec 16 2015

Fingerprint

Dopamine
Synapses
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Calcium
Isradipine
Calcium Channel Blockers
Zebrafish
Inner Ear
Synaptic Transmission
Hearing
Epithelium

Keywords

  • D1 receptor
  • Dopamine
  • Efferents
  • Hair cell
  • Lateral-line organ
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Toro, C., Trapani, J. G., Pacentine, I., Maeda, R., Sheets, L., Mo, W., & Nicolson, T. (2015). Dopamine modulates the activity of sensory hair cells. Journal of Neuroscience, 35(50), 16494-16503. https://doi.org/10.1523/JNEUROSCI.1691-15.2015

Dopamine modulates the activity of sensory hair cells. / Toro, Cecilia; Trapani, Josef G.; Pacentine, Itallia; Maeda, Reo; Sheets, Lavinia; Mo, Weike; Nicolson, Teresa.

In: Journal of Neuroscience, Vol. 35, No. 50, 16.12.2015, p. 16494-16503.

Research output: Contribution to journalArticle

Toro, C, Trapani, JG, Pacentine, I, Maeda, R, Sheets, L, Mo, W & Nicolson, T 2015, 'Dopamine modulates the activity of sensory hair cells', Journal of Neuroscience, vol. 35, no. 50, pp. 16494-16503. https://doi.org/10.1523/JNEUROSCI.1691-15.2015
Toro C, Trapani JG, Pacentine I, Maeda R, Sheets L, Mo W et al. Dopamine modulates the activity of sensory hair cells. Journal of Neuroscience. 2015 Dec 16;35(50):16494-16503. https://doi.org/10.1523/JNEUROSCI.1691-15.2015
Toro, Cecilia ; Trapani, Josef G. ; Pacentine, Itallia ; Maeda, Reo ; Sheets, Lavinia ; Mo, Weike ; Nicolson, Teresa. / Dopamine modulates the activity of sensory hair cells. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 50. pp. 16494-16503.
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