Dopamine Autoreceptor Stimulation Increases Protein Carboxyl Methylation in Striatal Slices

Marina E. Wolf, Robert H. Roth

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We have investigated the possibility that protein carboxyl methylation is involved in coupling dopamine autoreceptor stimulation to intracellular events such as inhibition of dopamine synthesis or release. The dopamine agonists apomorphine and TL‐99 were found to stimulate methyl ester formation in striatal slices preloaded with [3H]methionine. The stimulatory effects of apomorphine were dose‐dependent, were not due to changes in [3H]methionine uptake or S‐[3H]‐adenosyl‐methionine formation, and were blocked by the stereospecific dopamine antagonist (+)‐butaclamol. Stimulation of methyl ester formation by dopamine agonists is readily observed only when slices are prepared from rats pretreated with reserpine to deplete endogenous brain catecholamines. This suggests that in slices prepared from normal rats endogenous dopamine (DA) released during slice preparation and incubation masks the effects produced by exogenously administered dopamine agonists on protein carboxyl methylase (PCM) activity. Additional experiments suggested that the effects of apomorphine were mediated via an interaction with DA autoreceptors rather than with postsynaptic DA receptors. Destruction of monoamine neurons and their associated autoreceptors by injecting 6‐hydroxydopamine into the area of the medial forebrain bundle abolished the stimulatory effects of apomorphine on methyl ester formation in striatal slices. Furthermore the putative selective DA autoreceptor agonist EMD 23 448 was also found to stimulate methyl ester formation in striatal slices. These findings, discussed in terms of calcium‐dependent functions, support the hypothesis that PCM may be a key component in the biochemical transduction of DA autoreceptor stimulation.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalJournal of neurochemistry
Volume44
Issue number1
DOIs
StatePublished - Jan 1985

Keywords

  • Brain slices
  • Dopamine autoreceptors
  • Protein carboxyl methylation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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