Dopamine and norepinephrine stimulate somatostatin release by median eminence fragments in vitro

A. Negro-Vilar, S. R. Ojeda, A. Arimura, S. M. McCann

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

The effect of catecholamines on somatostatin release by median eminence (ME) fragments was evaluated using an in vitro incubation system. Adult male rats were used as tissue donors. Somatostatin release was readily detected during short-term incubations (10 and 30 minutes). Dopamine (DA) significantly stimulated somatostatin release during a 30 minute incubation period at the two doses tested (0.6 and 6 μM). Under similar conditions, norepinephrine (NE) stimulated somatostatin release only at the 6 μM dose. Using a shorter incubation period (10 min) and a 6 μM dose, only DA stimulated somatostatin release. The effects of DA and NE were specifically blocked by the in vitro addition of pimozide or phentolamine, respectively, suggesting that dopaminergic and noradrenergic receptors may be present in the somatostatinergic terminals of the ME. The results indicate that both DA and NE may be involved in the regulation of somatostatin secretion.

Original languageEnglish (US)
Pages (from-to)1493-1497
Number of pages5
JournalLife Sciences
Volume23
Issue number14
DOIs
StatePublished - Oct 9 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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