Dopamine and norepinephrine stimulate somatostatin release by median eminence fragments in vitro

A. Negro-Vilar, Sergio Ojeda, A. Arimura, S. M. McCann

Research output: Contribution to journalArticle

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Abstract

The effect of catecholamines on somatostatin release by median eminence (ME) fragments was evaluated using an in vitro incubation system. Adult male rats were used as tissue donors. Somatostatin release was readily detected during short-term incubations (10 and 30 minutes). Dopamine (DA) significantly stimulated somatostatin release during a 30 minute incubation period at the two doses tested (0.6 and 6 μM). Under similar conditions, norepinephrine (NE) stimulated somatostatin release only at the 6 μM dose. Using a shorter incubation period (10 min) and a 6 μM dose, only DA stimulated somatostatin release. The effects of DA and NE were specifically blocked by the in vitro addition of pimozide or phentolamine, respectively, suggesting that dopaminergic and noradrenergic receptors may be present in the somatostatinergic terminals of the ME. The results indicate that both DA and NE may be involved in the regulation of somatostatin secretion.

Original languageEnglish (US)
Pages (from-to)1493-1497
Number of pages5
JournalLife Sciences
Volume23
Issue number14
DOIs
StatePublished - Oct 9 1978
Externally publishedYes

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Median Eminence
Somatostatin
Dopamine
Norepinephrine
Pimozide
Phentolamine
Catecholamines
In Vitro Techniques
Rats
Tissue Donors
Tissue

ASJC Scopus subject areas

  • Pharmacology

Cite this

Dopamine and norepinephrine stimulate somatostatin release by median eminence fragments in vitro. / Negro-Vilar, A.; Ojeda, Sergio; Arimura, A.; McCann, S. M.

In: Life Sciences, Vol. 23, No. 14, 09.10.1978, p. 1493-1497.

Research output: Contribution to journalArticle

Negro-Vilar, A. ; Ojeda, Sergio ; Arimura, A. ; McCann, S. M. / Dopamine and norepinephrine stimulate somatostatin release by median eminence fragments in vitro. In: Life Sciences. 1978 ; Vol. 23, No. 14. pp. 1493-1497.
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