Dopamine and norepinephrine stimulate somatostatin release by median eminence fragments in vitro

A. Negro-Vilar, S. R. Ojeda, A. Arimura, S. M. McCann

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    85 Scopus citations


    The effect of catecholamines on somatostatin release by median eminence (ME) fragments was evaluated using an in vitro incubation system. Adult male rats were used as tissue donors. Somatostatin release was readily detected during short-term incubations (10 and 30 minutes). Dopamine (DA) significantly stimulated somatostatin release during a 30 minute incubation period at the two doses tested (0.6 and 6 μM). Under similar conditions, norepinephrine (NE) stimulated somatostatin release only at the 6 μM dose. Using a shorter incubation period (10 min) and a 6 μM dose, only DA stimulated somatostatin release. The effects of DA and NE were specifically blocked by the in vitro addition of pimozide or phentolamine, respectively, suggesting that dopaminergic and noradrenergic receptors may be present in the somatostatinergic terminals of the ME. The results indicate that both DA and NE may be involved in the regulation of somatostatin secretion.

    Original languageEnglish (US)
    Pages (from-to)1493-1497
    Number of pages5
    JournalLife Sciences
    Issue number14
    StatePublished - Oct 9 1978


    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology, Toxicology and Pharmaceutics(all)

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