Dominant TCR Vα usage by virus and tumor-reactive T cells with wide affinity ranges for their specific antigens

Lydie Trautmann, Nathalie Labarrière, Francine Jotereau, Vaios Karanikas, Nadine Gervois, Thierry Connerotte, Pierre Coulie, Marc Bonneville

Research output: Contribution to journalReview articlepeer-review

51 Scopus citations

Abstract

We have studied the TCR features and functional responses of three sets of human cytolytic T cell (CTL) clones, recognizing antigenic peptides presented by HLA-A2 and derived from the Epstein-Barr virus proteins BMLF1 and BRLF1 and from the melanoma protein Melan-A/MART-1. Within each set, a majority of clones used a recurrent Vα region, even though they expressed highly diverse TCR β chains and V(D)J junctional sequences. Functional assays and peptide/MHC multimer binding studies indicated that this restricted Vα usage was not associated with the affinity/avidity of the CTL clones. The Vα dominance, which may be a frequent feature of antigen-specific T cells, likely reflects a restricted geometry of TCR/peptide/MHC complexes, primarily determined by Vα CDR.

Original languageEnglish (US)
Pages (from-to)3181-3190
Number of pages10
JournalEuropean Journal of Immunology
Volume32
Issue number11
DOIs
StatePublished - Nov 2002
Externally publishedYes

Keywords

  • Epstein-Barr virus
  • HCMV
  • Melanoma
  • T lymphocyte
  • TCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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