Abstract
We have studied the TCR features and functional responses of three sets of human cytolytic T cell (CTL) clones, recognizing antigenic peptides presented by HLA-A2 and derived from the Epstein-Barr virus proteins BMLF1 and BRLF1 and from the melanoma protein Melan-A/MART-1. Within each set, a majority of clones used a recurrent Vα region, even though they expressed highly diverse TCR β chains and V(D)J junctional sequences. Functional assays and peptide/MHC multimer binding studies indicated that this restricted Vα usage was not associated with the affinity/avidity of the CTL clones. The Vα dominance, which may be a frequent feature of antigen-specific T cells, likely reflects a restricted geometry of TCR/peptide/MHC complexes, primarily determined by Vα CDR.
Original language | English (US) |
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Pages (from-to) | 3181-3190 |
Number of pages | 10 |
Journal | European Journal of Immunology |
Volume | 32 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2002 |
Externally published | Yes |
Keywords
- Epstein-Barr virus
- HCMV
- Melanoma
- T lymphocyte
- TCR
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology