Does cellular hydrogen peroxide diffuse or act locally?

Natalia M. Mishina, Pyotr A. Tyurin-Kuzmin, Kseniya N. Markvicheva, Alexander V. Vorotnikov, Vsevolod A. Tkachuk, Vibor Laketa, Carsten Schultz, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journalReview article

106 Citations (Scopus)

Abstract

Understanding of redox signaling requires data on the spatiotemporal distribution of hydrogen peroxide (H 2O 2) within the cell. The fluorescent reporter HyPer is a powerful instrument for H 2O 2 imaging. However, rapid diffusion of HyPer throughout the nucleocytoplasmic compartment does not allow visualization of H 2O 2 gradients on the micrometer scale. Here we dramatically improved the spatial resolution of H 2O 2 imaging by applying subcytoplasmic targeting of HyPer. The membrane-attached reporters identified "microdomains" of elevated H 2O 2 levels within the cytoplasm of the cells exposed to growth factors. We demonstrate that diffusion of H 2O 2 across the cytoplasm was strongly limited, providing evidence that H 2O 2 acts locally inside cells.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalAntioxidants and Redox Signaling
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

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Hydrogen Peroxide
Imaging techniques
Cytoplasm
Intercellular Signaling Peptides and Proteins
Visualization
Membranes
Oxidation-Reduction

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Mishina, N. M., Tyurin-Kuzmin, P. A., Markvicheva, K. N., Vorotnikov, A. V., Tkachuk, V. A., Laketa, V., ... Belousov, V. V. (2011). Does cellular hydrogen peroxide diffuse or act locally? Antioxidants and Redox Signaling, 14(1), 1-7. https://doi.org/10.1089/ars.2010.3539

Does cellular hydrogen peroxide diffuse or act locally? / Mishina, Natalia M.; Tyurin-Kuzmin, Pyotr A.; Markvicheva, Kseniya N.; Vorotnikov, Alexander V.; Tkachuk, Vsevolod A.; Laketa, Vibor; Schultz, Carsten; Lukyanov, Sergey; Belousov, Vsevolod V.

In: Antioxidants and Redox Signaling, Vol. 14, No. 1, 01.01.2011, p. 1-7.

Research output: Contribution to journalReview article

Mishina, NM, Tyurin-Kuzmin, PA, Markvicheva, KN, Vorotnikov, AV, Tkachuk, VA, Laketa, V, Schultz, C, Lukyanov, S & Belousov, VV 2011, 'Does cellular hydrogen peroxide diffuse or act locally?', Antioxidants and Redox Signaling, vol. 14, no. 1, pp. 1-7. https://doi.org/10.1089/ars.2010.3539
Mishina NM, Tyurin-Kuzmin PA, Markvicheva KN, Vorotnikov AV, Tkachuk VA, Laketa V et al. Does cellular hydrogen peroxide diffuse or act locally? Antioxidants and Redox Signaling. 2011 Jan 1;14(1):1-7. https://doi.org/10.1089/ars.2010.3539
Mishina, Natalia M. ; Tyurin-Kuzmin, Pyotr A. ; Markvicheva, Kseniya N. ; Vorotnikov, Alexander V. ; Tkachuk, Vsevolod A. ; Laketa, Vibor ; Schultz, Carsten ; Lukyanov, Sergey ; Belousov, Vsevolod V. / Does cellular hydrogen peroxide diffuse or act locally?. In: Antioxidants and Redox Signaling. 2011 ; Vol. 14, No. 1. pp. 1-7.
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