The relationship between DNA polymerases α and δ are evaluated immunologically by monoclonal antibody specificity against DNA polymerase α and murine polyclonal antiserum against calf thymus DNA polymerase δ. DNA polymerase α and δ are found to be immunologically distinct. The structural relationship between the proliferating cell nuclear antigen (PCNA)-dependent calf DNA polymerase δ and DNA polymerase α from human and calf was analyzed by two-dimensional tryptic peptide mapping of the catalytic polypeptides. The results demonstrate that the catalytic polypeptides of the PCNA-dependent calf polymerase δ and DNA polymerase α are distinct, unrelated, and do not share any common structural determinants. The immunological and structural relationship between a recently identified PCNA-independent form of DNA polymerase δ from HeLa cells was also assessed. This PCNA-independently human polymerase δ was found to be immunologically unrelated to human polymerase α but to share some immunological and structural determinants with the PCNA-dependent calf thymus polymerase δ.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Biological Chemistry|
|State||Published - Jan 1 1989|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology