DNA polymerase III requirement for repair of DNA damage caused by methyl methanesulfonate and hydrogen peroxide.

M. E. Hagensee, S. K. Bryan, Robb Moses

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The pcbA1 mutation allows DNA replication dependent on DNA polymerase I at the restrictive temperature in polC(Ts) strains. Cells which carry pcbA1, a functional DNA polymerase I, and a temperature-sensitive DNA polymerase III gene were used to study the role of DNA polymerase III in DNA repair. At the restrictive temperature for DNA polymerase III, these strains were more sensitive to the alkylating agent methyl methanesulfonate (MMS) and hydrogen peroxide than normal cells. The same strains showed no increase in sensitivity to bleomycin, UV light, or psoralen at the restrictive temperature. The sensitivity of these strains to MMS and hydrogen peroxide was not due to the pcbAl allele, and normal sensitivity was restored by the introduction of a chromosomal or cloned DNA polymerase III gene, verifying that the sensitivity was due to loss of DNA polymerase III alpha-subunit activity. A functional DNA polymerase III is required for the reformation of high-molecular-weight DNA after treatment of cells with MMS or hydrogen peroxide, as demonstrated by alkaline sucrose sedimentation results. Thus, it appears that a functional DNA polymerase III is required for the optimal repair of DNA damage by MMS or hydrogen peroxide.

Original languageEnglish (US)
Pages (from-to)4608-4613
Number of pages6
JournalJournal of Bacteriology
Volume169
Issue number10
StatePublished - Oct 1987
Externally publishedYes

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DNA Polymerase III
Methyl Methanesulfonate
Hydrogen Peroxide
DNA Damage
DNA Polymerase I
Temperature
Ficusin
Alkylating Agents
Bleomycin
Ultraviolet Rays
DNA Replication
DNA Repair
Genes
Sucrose
Molecular Weight
Alleles
Mutation
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

DNA polymerase III requirement for repair of DNA damage caused by methyl methanesulfonate and hydrogen peroxide. / Hagensee, M. E.; Bryan, S. K.; Moses, Robb.

In: Journal of Bacteriology, Vol. 169, No. 10, 10.1987, p. 4608-4613.

Research output: Contribution to journalArticle

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