DNA polymerase III of Escherichia coli is required for UV and ethyl methanesulfonate mutagenesis

M. E. Hagensee, T. L. Timme, S. K. Bryan, Robb Moses

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Strains of Escherichia coli possessing the pcbA1 mutation, a functional DNA polymerase I, and a temperature-sensitive mutation in DNA polymerase III can survive at the restrictive temperature (43°C) for DNA polymerase III. The mutation rate of the bacterial genome of such strains after exposure to either UV light or ethyl melthanesulfonate was measured by its rifampicin resistance or amino acid requirements. In addition, Weigle mutagenesis of preirradiated λ phage was also measured. In all cases, no increase in mutagenesis was noted at the restrictive temperature for DNA polymerase III. Introduction of a cloned DNA polymerase III gene returned the mutation rate of the bacterial genome as well as the Weigle mutagenesis to normal at 43°C. Using a recA-lacZ fusion, the SOS response after UV irradiation was measured and found to be normal at the restrictive and permissive temperature for DNA polymerase III, as was induction of λ prophage. Recombination was also normal at either temperature. Our studies demonstrate that a functional DNA polymerse III is strictly required for mutagenesis at a step other than SOS induction.

Original languageEnglish (US)
Pages (from-to)4195-4199
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number12
StatePublished - 1987
Externally publishedYes

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DNA Polymerase III
Ethyl Methanesulfonate
Mutagenesis
Escherichia coli
Temperature
Bacterial Genomes
Mutation Rate
Virus Activation
DNA Polymerase I
Mutation
Rifampin
Ultraviolet Rays
Bacteriophages
Genetic Recombination
Amino Acids
DNA
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

DNA polymerase III of Escherichia coli is required for UV and ethyl methanesulfonate mutagenesis. / Hagensee, M. E.; Timme, T. L.; Bryan, S. K.; Moses, Robb.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 12, 1987, p. 4195-4199.

Research output: Contribution to journalArticle

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