DNA polymerase III-dependent repair synthesis in response to bleomycin in toluene-treated Escherichia coli

Steven L. Ross, Surendra Sharma, Robb Moses

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bleomycin (BLM) is an antitumor drug which interacts with and damages DNA. We have reported a repair response dependent on DNA polymerase I in toluene-treated Escherichia coli. We report here that DNA polymerase III can also catalyze a repair response in toluene-treated E. coli following exposure to BLM. Polymerase III-mediated synthesis differs because it is ATP-dependent, whereas polymerase I-mediated repair synthesis is not. Polymerase III repair synthesis is independent of replicative synthesis, as demonstrated in a polA-, dnaBts strain, or use of Novobiocin to inhibit replication, and replication persists in the presence of repair synthesis. It appears that ATP-dependent repair synthesis in response to BLM is also present in polA+ strains. Repair synthesis does not require the uvrA gene product.

Original languageEnglish (US)
Pages (from-to)595-605
Number of pages11
JournalMGG Molecular & General Genetics
Volume179
Issue number3
DOIs
StatePublished - Oct 1980
Externally publishedYes

Fingerprint

DNA Polymerase III
Bleomycin
Toluene
Escherichia coli
Adenosine Triphosphate
Novobiocin
DNA Polymerase I
Antineoplastic Agents
DNA Damage
Genes
indium-bleomycin

ASJC Scopus subject areas

  • Genetics

Cite this

DNA polymerase III-dependent repair synthesis in response to bleomycin in toluene-treated Escherichia coli. / Ross, Steven L.; Sharma, Surendra; Moses, Robb.

In: MGG Molecular & General Genetics, Vol. 179, No. 3, 10.1980, p. 595-605.

Research output: Contribution to journalArticle

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