DNA ploidy and cell cycle analysis in ear malignant melanoma by flow and image cytometry

C. Cohen, B. F. Walker, Alvin Jr Solomon, P. B. DeRose

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Sixteen ear malignant melanomas (MM) were studied for ploidy and cell cycle analysis by flow and image cytometry. The results were compared with clinical (age, sex, stage), histologic (depth of invasion, level, type) and prognostic (recurrence, death) parameters. Single nuclear suspensions were obtained from fixed, paraffin-embedded tumor and adjacent normal tissue processed separately according to Hedley's technique. These, a 'spiked' specimen of normal tissue and tumor, and a spleen diploid control were analyzed on a FACScan flow cytometer (Becton Dickinson, Mountain View, California, U.S.A.). Feulgen-stained Cytocentrifuge preparations of nuclear suspensions of normal, MM and diploid spleen were analyzed with the CAS 200 Image Analyzer (Cell Analysis Systems, Inc., Elmhurst, Illinois, U.S.A.) against commercial calibration rat hepatocytes defined as diploid. Six (37.5%) MM were diploid, and 10 (62.5%) were aneuploid; 8 (90%) were hypodiploid, for a high frequency. There were no statistically significant correlations between clinical, pathologic, prognostic or cell cycle analysis parameters and ploidy, although poor prognostic features tended to be in aneuploid lesions.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Volume14
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Image Cytometry
Ploidies
Diploidy
Ear
Melanoma
Cell Cycle
Flow Cytometry
DNA
Aneuploidy
Suspensions
Spleen
Paraffin
Calibration
Hepatocytes
Neoplasms
Recurrence

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology

Cite this

DNA ploidy and cell cycle analysis in ear malignant melanoma by flow and image cytometry. / Cohen, C.; Walker, B. F.; Solomon, Alvin Jr; DeRose, P. B.

In: Analytical and Quantitative Cytology and Histology, Vol. 14, No. 2, 1992, p. 81-88.

Research output: Contribution to journalArticle

Cohen, C. ; Walker, B. F. ; Solomon, Alvin Jr ; DeRose, P. B. / DNA ploidy and cell cycle analysis in ear malignant melanoma by flow and image cytometry. In: Analytical and Quantitative Cytology and Histology. 1992 ; Vol. 14, No. 2. pp. 81-88.
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