DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice

Viktoriya Dubrovskaya, A. S. Andryushkova, I. A. Kuznetsova, L. B. Toporkova, V. N. Buneva, I. A. Orlovskaya, G. A. Nevinsky

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Catalytically active antibodies (abzymes) hydrolyzing proteins, polysaccharides, ATP, DNA, and RNA have been detected in the sera of patients with various autoimmune and some viral diseases, but abzymes from the sera of animals are practically unstudied. The development of lupus-like autoimmune disease of MRL/MpJ-lpr mice is an experimental model for study of autoimmune pathologies and immunopathogenesis. In this work, homogeneous IgG preparations were isolated from the sera of MRL/MpJ-lpr mice. These antibodies (Abs), their Fab-fragments, and isolated light chains were shown to possess catalytic activity in DNA hydrolysis, whereas Abs from the sera of control healthy mice did not hydrolyze DNA. The data demonstrate that DNA hydrolyzing activity is an intrinsic property of Abs from MRL/MpJ-lpr mice. It was shown that various markers of autoimmune pathologies (level of total protein concentration in urea (proteinuria), Abs titers to native and denatured DNA, and DNA-hydrolyzing activity of IgG) increased in animals with aging, but they noticeably increased (2-22 times) only after appearance of obvious indicators of pathology independently of age. The highest increase in proteinuria (25-fold), anti-DNA Abs titers (12-19-fold), and abzyme activity (120-fold) was found in mice after their immunization with DNA-protein complex.

Original languageEnglish (US)
Pages (from-to)1081-1088
Number of pages8
JournalBiochemistry (Moscow)
Volume68
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Inbred MRL lpr Mouse
Antibodies
DNA
Serum
Pathology
Proteinuria
Animals
Immunoglobulin G
Immunization
Immunoglobulin Fab Fragments
Proteins
Antinuclear Antibodies
Virus Diseases
Autoimmune Diseases
Polysaccharides
Urea
Hydrolysis
Catalyst activity
Theoretical Models
Adenosine Triphosphate

Keywords

  • DNA hydrolysis
  • Immunoglobulins
  • MRL/MpJ-lpr mice sera
  • Native abzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dubrovskaya, V., Andryushkova, A. S., Kuznetsova, I. A., Toporkova, L. B., Buneva, V. N., Orlovskaya, I. A., & Nevinsky, G. A. (2003). DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice. Biochemistry (Moscow), 68(10), 1081-1088. https://doi.org/10.1023/A:1026350426842

DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice. / Dubrovskaya, Viktoriya; Andryushkova, A. S.; Kuznetsova, I. A.; Toporkova, L. B.; Buneva, V. N.; Orlovskaya, I. A.; Nevinsky, G. A.

In: Biochemistry (Moscow), Vol. 68, No. 10, 01.10.2003, p. 1081-1088.

Research output: Contribution to journalArticle

Dubrovskaya, V, Andryushkova, AS, Kuznetsova, IA, Toporkova, LB, Buneva, VN, Orlovskaya, IA & Nevinsky, GA 2003, 'DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice', Biochemistry (Moscow), vol. 68, no. 10, pp. 1081-1088. https://doi.org/10.1023/A:1026350426842
Dubrovskaya V, Andryushkova AS, Kuznetsova IA, Toporkova LB, Buneva VN, Orlovskaya IA et al. DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice. Biochemistry (Moscow). 2003 Oct 1;68(10):1081-1088. https://doi.org/10.1023/A:1026350426842
Dubrovskaya, Viktoriya ; Andryushkova, A. S. ; Kuznetsova, I. A. ; Toporkova, L. B. ; Buneva, V. N. ; Orlovskaya, I. A. ; Nevinsky, G. A. / DNA-Hydrolyzing Antibodies from Sera of Autoimmune-Prone MRL/MpJ-lpr Mice. In: Biochemistry (Moscow). 2003 ; Vol. 68, No. 10. pp. 1081-1088.
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