DNA determination in dysplastic nevi: A comparative study between flow cytometry and image analysis

O. P. Sangueza, D. M. Hyder, A. C. Bakke, C. R. White

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Dysplastic nevi (DN), described in 1978, have been associated with increased risk of melanoma, but the role of DN as precursors of melanoma is still controversial. Recent studies have shown that DN are very common in the general population, bringing into question this purported association. Numerous investigations have attempted to correlate the presence of DN in individuals with specific phenotypic and genotypic features, including the presence of abnormal DNA content. Because the occurrence of such abnormal DNA stemlines in neoplasms may be associated with malignant behavior, we studied 38 biopsies from 19 patients that histologically fulfilled criteria for DN in order to ascertain characteristics of DNA content. Nuclear suspensions made from paraffin-embedded tissue were evaluated by both flow cytometry and image analysis techniques. All cases demonstrated diploid populations by both DNA measurement methods. Our results contradict previous reports of aneuploid populations in these melanocytic lesions.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalAmerican Journal of Dermatopathology
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1993

Keywords

  • DNA determination
  • Dysplastic nevus
  • Flow cytometry
  • Image analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dermatology

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