DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine

C. Cremer, J. W. Gray

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Asynchronously growing Chinese hamster cells (M3-1) were UV-irradiated (λ = 254 nm) and then incubated with/without caffeine (2 mM) for 20 h. Microscopic evaluation of metaphase spreads revealed that after UV-irradiation alone (5.0 J/m2) and caffeine treatment alone, the percentage of cells with condensed chromatin appearing fragmented and/or pulverized ('GCS-like' cells; GCS, Generalized Chromosome Shattering) was very low while it was high following the combined treatment. Cytogenetic and flow cytometric analysis of cells obtained by mechanical shaking cultures treated with UV and caffeine indicated that 'GCS-like' cells have the same DNA content as untreated cells in G2 phase and mitosis.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume94
Issue number1
DOIs
StatePublished - May 1982

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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