DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine

C. Cremer; J. W. Gray

doi:10.1016/0027-5107(82)90175-0

DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine

C. Cremer, J. W. Gray

Research output: Contribution to journal › Article › peer-review

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Abstract

Asynchronously growing Chinese hamster cells (M3-1) were UV-irradiated (λ = 254 nm) and then incubated with/without caffeine (2 mM) for 20 h. Microscopic evaluation of metaphase spreads revealed that after UV-irradiation alone (5.0 J/m²) and caffeine treatment alone, the percentage of cells with condensed chromatin appearing fragmented and/or pulverized ('GCS-like' cells; GCS, Generalized Chromosome Shattering) was very low while it was high following the combined treatment. Cytogenetic and flow cytometric analysis of cells obtained by mechanical shaking cultures treated with UV and caffeine indicated that 'GCS-like' cells have the same DNA content as untreated cells in G2 phase and mitosis.

Original language	English (US)
Pages (from-to)	133-142
Number of pages	10
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(82)90175-0
State	Published - May 1982
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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title = "DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine",

abstract = "Asynchronously growing Chinese hamster cells (M3-1) were UV-irradiated (λ = 254 nm) and then incubated with/without caffeine (2 mM) for 20 h. Microscopic evaluation of metaphase spreads revealed that after UV-irradiation alone (5.0 J/m2) and caffeine treatment alone, the percentage of cells with condensed chromatin appearing fragmented and/or pulverized ('GCS-like' cells; GCS, Generalized Chromosome Shattering) was very low while it was high following the combined treatment. Cytogenetic and flow cytometric analysis of cells obtained by mechanical shaking cultures treated with UV and caffeine indicated that 'GCS-like' cells have the same DNA content as untreated cells in G2 phase and mitosis.",

author = "C. Cremer and Gray, {J. W.}",

note = "Funding Information: Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48, with support from the Deutsche Forschungsgemeinschaft (Cr 60/1 and Cr 60/2-2) and USPHS Grant A-14533 (J.G.). Funding Information: * On leave from the Institute of Human Genetics, University of Freiburg, D-7800 Freiburg i. Br., Fed. Rep. of Germany. Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48.",

year = "1982",

month = may,

doi = "10.1016/0027-5107(82)90175-0",

language = "English (US)",

volume = "94",

pages = "133--142",

journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

issn = "0027-5107",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine

AU - Cremer, C.

AU - Gray, J. W.

N1 - Funding Information: Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48, with support from the Deutsche Forschungsgemeinschaft (Cr 60/1 and Cr 60/2-2) and USPHS Grant A-14533 (J.G.). Funding Information: * On leave from the Institute of Human Genetics, University of Freiburg, D-7800 Freiburg i. Br., Fed. Rep. of Germany. Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48.

PY - 1982/5

Y1 - 1982/5

N2 - Asynchronously growing Chinese hamster cells (M3-1) were UV-irradiated (λ = 254 nm) and then incubated with/without caffeine (2 mM) for 20 h. Microscopic evaluation of metaphase spreads revealed that after UV-irradiation alone (5.0 J/m2) and caffeine treatment alone, the percentage of cells with condensed chromatin appearing fragmented and/or pulverized ('GCS-like' cells; GCS, Generalized Chromosome Shattering) was very low while it was high following the combined treatment. Cytogenetic and flow cytometric analysis of cells obtained by mechanical shaking cultures treated with UV and caffeine indicated that 'GCS-like' cells have the same DNA content as untreated cells in G2 phase and mitosis.

AB - Asynchronously growing Chinese hamster cells (M3-1) were UV-irradiated (λ = 254 nm) and then incubated with/without caffeine (2 mM) for 20 h. Microscopic evaluation of metaphase spreads revealed that after UV-irradiation alone (5.0 J/m2) and caffeine treatment alone, the percentage of cells with condensed chromatin appearing fragmented and/or pulverized ('GCS-like' cells; GCS, Generalized Chromosome Shattering) was very low while it was high following the combined treatment. Cytogenetic and flow cytometric analysis of cells obtained by mechanical shaking cultures treated with UV and caffeine indicated that 'GCS-like' cells have the same DNA content as untreated cells in G2 phase and mitosis.

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JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

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IS - 1

ER -

DNA content of cells with generalized chromosome shattering induced by ultraviolet light plus caffeine

Abstract

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