Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin

Kazumasa Wakamatsu, Renny Kavanagh, Ana L. Kadekaro, Silva Terzieva, Richard A. Sturm, Sancy Leachman, Zalfa Abdel-Malek, Shosuke Ito

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Cultured human melanocytes differ tremendously in visual pigmentation, and recapitulate the pigmentary phenotype of the donor's skin. This diversity arises from variation in type as well as quantity of melanin produced. Here, we measured contents of eumelanin (EM) and pheomelanin (PM) in 60 primary human melanocyte cultures (51 neonatal and nine adults), and correlated some of these values with the respective activity and protein levels of tyrosinase, and the melanocortin-1 receptor (MC1R) genotype. Melanocytes were classified into four phenotypes (L, L+, D, D+) as depicted by visual pigmentation using light microscopy, and by the pigmentary phenotype of the donor's skin. There were large differences in total melanin (TM) and EM, which increased progressively for L, L+, D and D+ melanocytes. TM content, the sum of EM and PM, showed a good correlation with TM measured spectrophotometrically, and with the activity and protein levels of tyrosinase. Log EM/PM ratio did not correlate with MC1R genotype. We conclude that: (i) EM consistently correlates with the visual phenotype; (ii) lighter melanocytes tend to be more pheomelanic in composition than darker melanocytes; (iii) in adult melanocyte cultures, EM correlates with the ethnic background of the donors (African-American > Indian > Caucasian); and (iv) MC1R loss-of-function mutations do not necessarily alter the phenotype of cultured melanocytes.

Original languageEnglish (US)
Pages (from-to)154-162
Number of pages9
JournalPigment Cell Research
Volume19
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

melanocytes
Melanocytes
Melanins
Pigmentation
melanin
eumelanin
pigmentation
Receptor, Melanocortin, Type 1
alpha-melanocyte-stimulating hormone
Phenotype
phenotype
Monophenol Monooxygenase
Skin
Tissue Donors
skin (animal)
receptors
Genotype
Optical microscopy
genotype
Proteins

Keywords

  • Eumelanin
  • Melanocortin-1 receptor
  • Melanocytes
  • Pheomelanin
  • Tyrosinase

ASJC Scopus subject areas

  • Cell Biology
  • Agronomy and Crop Science
  • Plant Science
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin. / Wakamatsu, Kazumasa; Kavanagh, Renny; Kadekaro, Ana L.; Terzieva, Silva; Sturm, Richard A.; Leachman, Sancy; Abdel-Malek, Zalfa; Ito, Shosuke.

In: Pigment Cell Research, Vol. 19, No. 2, 04.2006, p. 154-162.

Research output: Contribution to journalArticle

Wakamatsu, Kazumasa ; Kavanagh, Renny ; Kadekaro, Ana L. ; Terzieva, Silva ; Sturm, Richard A. ; Leachman, Sancy ; Abdel-Malek, Zalfa ; Ito, Shosuke. / Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin. In: Pigment Cell Research. 2006 ; Vol. 19, No. 2. pp. 154-162.
@article{59095ff6b4d243eaa13455b1a6778e1e,
title = "Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin",
abstract = "Cultured human melanocytes differ tremendously in visual pigmentation, and recapitulate the pigmentary phenotype of the donor's skin. This diversity arises from variation in type as well as quantity of melanin produced. Here, we measured contents of eumelanin (EM) and pheomelanin (PM) in 60 primary human melanocyte cultures (51 neonatal and nine adults), and correlated some of these values with the respective activity and protein levels of tyrosinase, and the melanocortin-1 receptor (MC1R) genotype. Melanocytes were classified into four phenotypes (L, L+, D, D+) as depicted by visual pigmentation using light microscopy, and by the pigmentary phenotype of the donor's skin. There were large differences in total melanin (TM) and EM, which increased progressively for L, L+, D and D+ melanocytes. TM content, the sum of EM and PM, showed a good correlation with TM measured spectrophotometrically, and with the activity and protein levels of tyrosinase. Log EM/PM ratio did not correlate with MC1R genotype. We conclude that: (i) EM consistently correlates with the visual phenotype; (ii) lighter melanocytes tend to be more pheomelanic in composition than darker melanocytes; (iii) in adult melanocyte cultures, EM correlates with the ethnic background of the donors (African-American > Indian > Caucasian); and (iv) MC1R loss-of-function mutations do not necessarily alter the phenotype of cultured melanocytes.",
keywords = "Eumelanin, Melanocortin-1 receptor, Melanocytes, Pheomelanin, Tyrosinase",
author = "Kazumasa Wakamatsu and Renny Kavanagh and Kadekaro, {Ana L.} and Silva Terzieva and Sturm, {Richard A.} and Sancy Leachman and Zalfa Abdel-Malek and Shosuke Ito",
year = "2006",
month = "4",
doi = "10.1111/j.1600-0749.2006.00293.x",
language = "English (US)",
volume = "19",
pages = "154--162",
journal = "Pigment Cell and Melanoma Research",
issn = "1755-1471",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Diversity of pigmentation in cultured human melanocytes is due to differences in the type as well as quantity of melanin

AU - Wakamatsu, Kazumasa

AU - Kavanagh, Renny

AU - Kadekaro, Ana L.

AU - Terzieva, Silva

AU - Sturm, Richard A.

AU - Leachman, Sancy

AU - Abdel-Malek, Zalfa

AU - Ito, Shosuke

PY - 2006/4

Y1 - 2006/4

N2 - Cultured human melanocytes differ tremendously in visual pigmentation, and recapitulate the pigmentary phenotype of the donor's skin. This diversity arises from variation in type as well as quantity of melanin produced. Here, we measured contents of eumelanin (EM) and pheomelanin (PM) in 60 primary human melanocyte cultures (51 neonatal and nine adults), and correlated some of these values with the respective activity and protein levels of tyrosinase, and the melanocortin-1 receptor (MC1R) genotype. Melanocytes were classified into four phenotypes (L, L+, D, D+) as depicted by visual pigmentation using light microscopy, and by the pigmentary phenotype of the donor's skin. There were large differences in total melanin (TM) and EM, which increased progressively for L, L+, D and D+ melanocytes. TM content, the sum of EM and PM, showed a good correlation with TM measured spectrophotometrically, and with the activity and protein levels of tyrosinase. Log EM/PM ratio did not correlate with MC1R genotype. We conclude that: (i) EM consistently correlates with the visual phenotype; (ii) lighter melanocytes tend to be more pheomelanic in composition than darker melanocytes; (iii) in adult melanocyte cultures, EM correlates with the ethnic background of the donors (African-American > Indian > Caucasian); and (iv) MC1R loss-of-function mutations do not necessarily alter the phenotype of cultured melanocytes.

AB - Cultured human melanocytes differ tremendously in visual pigmentation, and recapitulate the pigmentary phenotype of the donor's skin. This diversity arises from variation in type as well as quantity of melanin produced. Here, we measured contents of eumelanin (EM) and pheomelanin (PM) in 60 primary human melanocyte cultures (51 neonatal and nine adults), and correlated some of these values with the respective activity and protein levels of tyrosinase, and the melanocortin-1 receptor (MC1R) genotype. Melanocytes were classified into four phenotypes (L, L+, D, D+) as depicted by visual pigmentation using light microscopy, and by the pigmentary phenotype of the donor's skin. There were large differences in total melanin (TM) and EM, which increased progressively for L, L+, D and D+ melanocytes. TM content, the sum of EM and PM, showed a good correlation with TM measured spectrophotometrically, and with the activity and protein levels of tyrosinase. Log EM/PM ratio did not correlate with MC1R genotype. We conclude that: (i) EM consistently correlates with the visual phenotype; (ii) lighter melanocytes tend to be more pheomelanic in composition than darker melanocytes; (iii) in adult melanocyte cultures, EM correlates with the ethnic background of the donors (African-American > Indian > Caucasian); and (iv) MC1R loss-of-function mutations do not necessarily alter the phenotype of cultured melanocytes.

KW - Eumelanin

KW - Melanocortin-1 receptor

KW - Melanocytes

KW - Pheomelanin

KW - Tyrosinase

UR - http://www.scopus.com/inward/record.url?scp=33644847858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644847858&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0749.2006.00293.x

DO - 10.1111/j.1600-0749.2006.00293.x

M3 - Article

C2 - 16524431

AN - SCOPUS:33644847858

VL - 19

SP - 154

EP - 162

JO - Pigment Cell and Melanoma Research

JF - Pigment Cell and Melanoma Research

SN - 1755-1471

IS - 2

ER -