Distribution of mRNA encoding a novel opioid receptor in mouse brain

C. Saez, Q. Y. Zhou, O. Civelli, J. R. Bunzow, D. K. Grandy

    Research output: Contribution to journalArticle

    Abstract

    We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

    Original languageEnglish (US)
    Pages (from-to)S13-S14
    JournalRegulatory Peptides
    Volume53
    Issue numberSUPPL. 1
    DOIs
    StatePublished - Feb 21 1994

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Clinical Biochemistry
    • Cellular and Molecular Neuroscience

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  • Cite this

    Saez, C., Zhou, Q. Y., Civelli, O., Bunzow, J. R., & Grandy, D. K. (1994). Distribution of mRNA encoding a novel opioid receptor in mouse brain. Regulatory Peptides, 53(SUPPL. 1), S13-S14. https://doi.org/10.1016/0167-0115(94)90217-8