Distribution of mRNA encoding a novel opioid receptor in mouse brain

C. Saez, Q. Y. Zhou, O. Civelli, J. R. Bunzow, David Grandy

    Research output: Contribution to journalArticle

    Abstract

    We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

    Original languageEnglish (US)
    JournalRegulatory Peptides
    Volume53
    Issue numberSUPPL. 1
    DOIs
    StatePublished - Feb 21 1994

    Fingerprint

    Opioid Receptors
    Brain
    kappa Opioid Receptor
    Messenger RNA
    delta Opioid Receptor
    DNA Primers
    G-Protein-Coupled Receptors
    Rats
    Clone Cells
    Sequence Analysis
    Polymerase Chain Reaction

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Neuroscience(all)

    Cite this

    Saez, C., Zhou, Q. Y., Civelli, O., Bunzow, J. R., & Grandy, D. (1994). Distribution of mRNA encoding a novel opioid receptor in mouse brain. Regulatory Peptides, 53(SUPPL. 1). https://doi.org/10.1016/0167-0115(94)90217-8

    Distribution of mRNA encoding a novel opioid receptor in mouse brain. / Saez, C.; Zhou, Q. Y.; Civelli, O.; Bunzow, J. R.; Grandy, David.

    In: Regulatory Peptides, Vol. 53, No. SUPPL. 1, 21.02.1994.

    Research output: Contribution to journalArticle

    Saez, C, Zhou, QY, Civelli, O, Bunzow, JR & Grandy, D 1994, 'Distribution of mRNA encoding a novel opioid receptor in mouse brain', Regulatory Peptides, vol. 53, no. SUPPL. 1. https://doi.org/10.1016/0167-0115(94)90217-8
    Saez, C. ; Zhou, Q. Y. ; Civelli, O. ; Bunzow, J. R. ; Grandy, David. / Distribution of mRNA encoding a novel opioid receptor in mouse brain. In: Regulatory Peptides. 1994 ; Vol. 53, No. SUPPL. 1.
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