Distribution of estrogen receptor beta (ERβ) mRNA in hypothalamus, midbrain and temporal lobe of spayed macaque: Continued expression with hormone replacement

Chrisana Gundlah, Steven G. Kohama, Stephanie J. Mirkes, Vasilios T. Garyfallou, Henryk F. Urbanski, Cynthia L. Bethea

    Research output: Contribution to journalArticle

    170 Scopus citations

    Abstract

    This study used in situ hybridization (ISH) to examine the distribution of estrogen receptor beta (ERβ) mRNA in hypothalamic, limbic, and midbrain regions of monkey brain and its regulation by estrogen (E) and progesterone (P). Monkey-specific ERβ cDNAs were developed with human primers and reverse transcription and polymerase chain reaction (RT-PCR) using mRNA extracted from a rhesus monkey prostate gland. ERβ 5' (262 bases) and 3' (205 bases) riboprobes were used in combination for ISH. Ovariectomized and hysterectomized (spayed) pigtail macaques (Macaca nemestrina; four per treatment group) were either untreated spayed-controls, treated with E (28 days), or treated with E plus P (14 days E+14 days E and P). Dense ERβ hybridization signal was seen in the preoptic area, paraventricular nucleus, and ventromedial nucleus of the hypothalamus; the substantia nigra, caudal linear, dorsal raphe, and pontine nuclei of the midbrain; the dentate gyrus, CA1, CA2, CA3, CA4, and the prosubiculum/subiculum areas of the hippocampus. Expression in the suprachiasmatic region, supraoptic nucleus, arcuate nucleus, and amygdala was less intense. Image analysis of the dense areas showed no significant difference in the hybridization signal in individual regions of the hypothalamus, midbrain, or hippocampus between any of the treatment groups. However, P treatment decreased overall ERβ signal in the hypothalamus and hippocampus when several different subregions were combined. The localization of ERβ in monkey brain by ISH is in general agreement with that previously described in rodents. The presence of monkey ERβ mRNA in brain regions that lack ERα should help to clarify the molecular mechanisms by which E acts in the central nervous system to influence hormone secretion, mood disorders, cognition, and neuroprotection. Copyright (C) 2000 Elsevier Science B.V.

    Original languageEnglish (US)
    Pages (from-to)191-204
    Number of pages14
    JournalMolecular Brain Research
    Volume76
    Issue number2
    DOIs
    StatePublished - Mar 29 2000

    Keywords

    • Brain
    • Estrogen receptor beta
    • Hormone replacement therapy
    • Macaque
    • Ovarian steroid

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

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