TY - JOUR
T1 - Distribution of dopamine D1, D2, and D5 receptor mRNAs in the monkey brain
T2 - ribonuclease protection assay analysis
AU - Sung Choi, Wan
AU - Machida, Curtis A.
AU - Ronnekleiv, Oline K.
N1 - Funding Information:
This work was supported by NIH Grant DA-07165. The Population Center P30 program project Grant HD-18185 was used for steroid determinations, and the Animal Resources Branch Grant P51 RR-00163 for operation of the Oregon Regional Primate Research Center. C.A.M. is supported by an American Heart Association Established Investigatorship and by the NIH Grant P51 RR-00163. We thank Dr. David K. Grandy for the generous supply of human D5 receptor cDNA, and Dr. Julie A. Brown for technical advice. The technical assistance of Mr. Barry R. Naylor is gratefully acknowledged. This is publication no. 1951 of the Oregon Regional Primate Research Center.
PY - 1995/7
Y1 - 1995/7
N2 - The distribution of the mRNAs encoding the dopamine D1, D2 and D5 receptors was determined in brain tissues obtained from intact female rhesus monkeys, using a ribonuclease protection assay. Tissue blocks from the frontal cortex, striatum, thalamus, hippocampus and substantia nigra were dissected and total RNA was extracted. Dopamine D2 and D5 receptor DNA fragments were generated from rhesus monkey genomic DNA using polymerase chain reaction. To generate dopamine receptor subtype-specific cRNA probes, DNA fragments corresponding to the carboxy terminus of the rhesus monkey D1 and D2 receptor genes and to the putative transmembrane domain regions (IV-VI) of the D5 receptor gene, were subcloned into the pGEM3Z/4Z vectors. Expression of D1 receptor mRNA exhibited significant regional differences: striatum > > > cerebral cortex ≥ hippocampus ≥ lateral thalamus. D1 receptor mRNA was found in low quantities in the medial thalamus, but was not consistently expressed in the substantia nigra area. In contrast, D2 receptor mRNA was detected in all regions that were studied: striatum > > > substantia nigra > > hippocampus ≥ cerebral cortex ≥ medial thalamus ≥ lateral thalamus. D5 receptor mRNA was also expressed in all regions, with highest levels in the cerebral cortex, striatum and lateral thalamus, and moderate levels in the substantia nigra, medial thalamus and the hippocampus. The D5 receptor mRNA appears to be widely distributed in the monkey brain. Most interesting is the expression of D5 receptor mRNA in tissues of the substantia nigra area.
AB - The distribution of the mRNAs encoding the dopamine D1, D2 and D5 receptors was determined in brain tissues obtained from intact female rhesus monkeys, using a ribonuclease protection assay. Tissue blocks from the frontal cortex, striatum, thalamus, hippocampus and substantia nigra were dissected and total RNA was extracted. Dopamine D2 and D5 receptor DNA fragments were generated from rhesus monkey genomic DNA using polymerase chain reaction. To generate dopamine receptor subtype-specific cRNA probes, DNA fragments corresponding to the carboxy terminus of the rhesus monkey D1 and D2 receptor genes and to the putative transmembrane domain regions (IV-VI) of the D5 receptor gene, were subcloned into the pGEM3Z/4Z vectors. Expression of D1 receptor mRNA exhibited significant regional differences: striatum > > > cerebral cortex ≥ hippocampus ≥ lateral thalamus. D1 receptor mRNA was found in low quantities in the medial thalamus, but was not consistently expressed in the substantia nigra area. In contrast, D2 receptor mRNA was detected in all regions that were studied: striatum > > > substantia nigra > > hippocampus ≥ cerebral cortex ≥ medial thalamus ≥ lateral thalamus. D5 receptor mRNA was also expressed in all regions, with highest levels in the cerebral cortex, striatum and lateral thalamus, and moderate levels in the substantia nigra, medial thalamus and the hippocampus. The D5 receptor mRNA appears to be widely distributed in the monkey brain. Most interesting is the expression of D5 receptor mRNA in tissues of the substantia nigra area.
KW - Distribution
KW - Dopamine D1 receptor
KW - Dopamine D2 receptor
KW - Dopamine D5 receptor
KW - Monkey brain
KW - Ribonuclease protection assay
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U2 - 10.1016/0169-328X(95)00038-T
DO - 10.1016/0169-328X(95)00038-T
M3 - Article
C2 - 7476036
AN - SCOPUS:0029059384
SN - 0169-328X
VL - 31
SP - 86
EP - 94
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -