Distribution of cholinergic and dopaminergic receptors in rainbow trout pineal gland

Michikazu Samejima, H. Kevin Happe, L. Charles Murrin, Ronald F. Pfeiffer, Manuchair Ebadi

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Samejima M, Happe HK, Murrin LC, Pfeiffer RE, Ebadi M. Distribution of cholinergic and dopaminergic receptors in rainbow trout pineal gland. J. Pineal Res. 1994: 16: 37–43. The involvement of multiple receptors in modulating the function of the pineal gland was investigated by searching for dopaminergic and cholinergic receptors in trout pineal gland. Dopamine D1 and D2 receptors were measured using [3H]SCH23390 and [3H]spiperone, respectively. Muscarinic and nicotinic cholinergic receptors were measured using quinuclidinyl benzilate ([3H]QNB) and [3H]methylcarbamyl choline, respectively. High‐affinity choline uptake sites were measured using [3H]hemicholinium‐3. The distribution of dopaminergic receptors varied throughout the pineal gland in that the density of D2 receptors, which was higher than that of D1 receptors, was most abundant in the distal region, exhibiting a value of 112 ± 17 fmol/mg tissue. The distribution of both muscarinic and nicotinic receptors was uniform throughout the pineal gland. However, the highest value for the high‐affinity choline transporter (106 ± 17 fmol/mg tissue) occurred in the proximal portion of the trout pineal gland. The results of these studies indicate that the pineal gland should not be viewed as a homogenous tissue possessing identical density of various receptors. Furthermore, these results, along with previous data, are interpreted to suggest that different regions of pineal gland may indeed possess unique functions.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalJournal of pineal research
Volume16
Issue number1
DOIs
StatePublished - Jan 1994

Keywords

  • dopamine D and D receptors
  • high‐affinity choline uptake sites
  • muscarinic cholinergic receptors
  • nicotinic cholinergic receptors
  • pineal gland
  • trout

ASJC Scopus subject areas

  • Endocrinology

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