Distribution of aromatase mRNA in the ram hypothalamus: An in situ hybridization study

Charles E. Roselli, F. Stormshak, J. A. Resko

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The regional distribution of neurones expressing aromatase mRNA in the ram hypothalamus was examined by in situ hybridization using 33P-labelled cRNA probes. The highest amounts of hybridization signal were observed in the central part of the medial preoptic nucleus and posterior medial part of the bed nucleus of the stria terminalis. Moderate amounts of hybridization signal were observed in the anteroventral periventricular preoptic nucleus, medial preoptic nucleus and a broad band extending between the medial preoptic nucleus and bed nucleus of the stria terminalis. Low levels of hybridization signal were observed in the organum vasculosum of the lamina terminalis, anterior part of the medial preoptic nucleus, and central part of the ventromedial nucleus of the hypothalamus. The presence of aromatase mRNA within neurones of the steroid-sensitive hypothalamic circuit supports a role for aromatization in the mechanism of testosterone action on reproductive function in male sheep. The distribution of aromatase mRNA in the ovine hypothalamus was similar to that described for other vertebrate species, suggesting a high degree of functional conservation across species.

Original languageEnglish (US)
Pages (from-to)656-664
Number of pages9
JournalJournal of Neuroendocrinology
Volume12
Issue number7
DOIs
StatePublished - Jun 24 2000

Keywords

  • CYP19
  • Cytochrome P450 aromatase
  • Hypothalamus
  • In situ hybridization
  • Preoptic area

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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