TY - JOUR
T1 - Distribution and Regulation of Rat Insulin-Like Growth Factor I Messenger Ribonucleic Acids Encoding Alternative Carboxyterminal E-Peptides
T2 - Evidence for Differential Processing and Regulation in Liver
AU - Lowe, William L.
AU - Lasky, Stephen R.
AU - Leroith, Derek
AU - Roberts, Charles T.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/6
Y1 - 1988/6
N2 - Alternative splicing of insulin-like growth factor I (IGF-l)/somatomedin C mRNAs generates two IGF-I mRNAs coding for IGF-I peptides with different sequences in the E domain of the IGF-I prohormone. These two mRNAs encode alternative E peptides due to the presence (IGF-lb) or absence (IGF-la) of a 52-base insert in the region coding for the E domain. We have used a solution hybridization/RNase protection assay to determine the tissue distribution and regulation by GH of the expression of these alternative IGF-I mRNAs. IGF-lb mRNAs are present in low abundance (representing ~2-5% of the total IGF-I mRNA) in heart, lung, muscle, testes, stomach, kidney, and brain, but represent approximately 13% of the IGF-I mRNA in liver. GH treatment of hypophysectomized rats increased steady-state IGF-I mRNA levels in liver, kidney, lung, and heart. In kidney, lung, and heart, IGF-la and IGF-lb mRNA levels were coordinately regulated by GH, but, in liver, the fold increase in IGF-lb mRNA levels was approximately three times greater than the fold increase in IGF-la mRNA levels. These data suggest that the processing of IGF-I mRNA in liver is different than in nonhepatic tissues. These results also further elucidate the organization of the rat IGF-I gene as well as the generation of multiple IGF-I mRNAs by alternative splicing.
AB - Alternative splicing of insulin-like growth factor I (IGF-l)/somatomedin C mRNAs generates two IGF-I mRNAs coding for IGF-I peptides with different sequences in the E domain of the IGF-I prohormone. These two mRNAs encode alternative E peptides due to the presence (IGF-lb) or absence (IGF-la) of a 52-base insert in the region coding for the E domain. We have used a solution hybridization/RNase protection assay to determine the tissue distribution and regulation by GH of the expression of these alternative IGF-I mRNAs. IGF-lb mRNAs are present in low abundance (representing ~2-5% of the total IGF-I mRNA) in heart, lung, muscle, testes, stomach, kidney, and brain, but represent approximately 13% of the IGF-I mRNA in liver. GH treatment of hypophysectomized rats increased steady-state IGF-I mRNA levels in liver, kidney, lung, and heart. In kidney, lung, and heart, IGF-la and IGF-lb mRNA levels were coordinately regulated by GH, but, in liver, the fold increase in IGF-lb mRNA levels was approximately three times greater than the fold increase in IGF-la mRNA levels. These data suggest that the processing of IGF-I mRNA in liver is different than in nonhepatic tissues. These results also further elucidate the organization of the rat IGF-I gene as well as the generation of multiple IGF-I mRNAs by alternative splicing.
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U2 - 10.1210/mend-2-6-528
DO - 10.1210/mend-2-6-528
M3 - Article
C2 - 3419435
AN - SCOPUS:0023928237
SN - 0888-8809
VL - 2
SP - 528
EP - 535
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 6
ER -