Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse

Anders Juréus, Matthew J. Cunningham, Dorothy Li, Laura L. Johnson, Stephanie Krasnow, Dawit N. Teklemichael, Donald K. Clifton, Robert A. Steiner

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Galanin-like peptide (GALP) is a newly discovered molecule whose expression in the brain is confined to the arcuate nucleus and median eminence. In the rat, cellular levels of GALP mRNA are reduced by fasting and reversed by peripheral administration of leptin. The purpose of this investigation was 1) to clone and map the distribution of GALP mRNA in the brain of the mouse; 2) to compare the pattern and magnitude of GALP mRNA expression in the leptin-deficient obese (ob/ob) mouse with that of wild-type controls; and 3) to examine the effects of leptin delivered into the brain on the expression of GALP mRNA in the ob/ob mouse. We report the sequence of a mouse GALP cDNA and show that GALP mRNA is expressed in the arcuate nucleus, median eminence, infundibular stalk, and the neurohypophysis of this species. The expression of GALP mRNA in the brain was markedly reduced in the ob/ob mice, compared with wild-type animals. Intracerebroventricular infusion of leptin to ob/ob mice increased both the number of GALP mRNA-expressing neurons and their content of GALP mRNA, compared with vehicle-treated controls. These observations demonstrate that GALP mRNA is induced by leptin through a direct action on the brain.

Original languageEnglish (US)
Pages (from-to)5140-5144
Number of pages5
JournalEndocrinology
Volume142
Issue number12
DOIs
StatePublished - 2001
Externally publishedYes

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Galanin-Like Peptide
Hypothalamus
Messenger RNA
Leptin
Brain
Median Eminence
Arcuate Nucleus of Hypothalamus
Intraventricular Infusions
Posterior Pituitary Gland
Wild Animals
Pituitary Gland

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Juréus, A., Cunningham, M. J., Li, D., Johnson, L. L., Krasnow, S., Teklemichael, D. N., ... Steiner, R. A. (2001). Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. Endocrinology, 142(12), 5140-5144. https://doi.org/10.1210/en.142.12.5140

Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. / Juréus, Anders; Cunningham, Matthew J.; Li, Dorothy; Johnson, Laura L.; Krasnow, Stephanie; Teklemichael, Dawit N.; Clifton, Donald K.; Steiner, Robert A.

In: Endocrinology, Vol. 142, No. 12, 2001, p. 5140-5144.

Research output: Contribution to journalArticle

Juréus, A, Cunningham, MJ, Li, D, Johnson, LL, Krasnow, S, Teklemichael, DN, Clifton, DK & Steiner, RA 2001, 'Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse', Endocrinology, vol. 142, no. 12, pp. 5140-5144. https://doi.org/10.1210/en.142.12.5140
Juréus A, Cunningham MJ, Li D, Johnson LL, Krasnow S, Teklemichael DN et al. Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. Endocrinology. 2001;142(12):5140-5144. https://doi.org/10.1210/en.142.12.5140
Juréus, Anders ; Cunningham, Matthew J. ; Li, Dorothy ; Johnson, Laura L. ; Krasnow, Stephanie ; Teklemichael, Dawit N. ; Clifton, Donald K. ; Steiner, Robert A. / Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. In: Endocrinology. 2001 ; Vol. 142, No. 12. pp. 5140-5144.
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