Distinct muscarinic receptors inhibit release of γ-aminobutyric acid and excitatory amino acids in mammalian brain

S. Sugita, N. Uchimura, Zhi-Gen Jiang, R. A. North

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Intracellular recordings were made from neurons of rat lateral amygdala, nucleus accumbens, and striatum in vitro. Synaptic potentials mediated by γ-aminobutyric acid and by excitatory amino acids were isolated pharmacologically by using receptor antagonists, and their amplitudes were used as a measure of transmitter release. Muscarine and acetylcholine inhibited the release of both γ-aminobutyric acid and excitatory amino acids, but measurements of the dissociation equilibrium constants for the antagonists pirenzepine, 11-{2-[(diethylamino)methyl]-1-piperidinyl}acetyl-5,11-dihydro-6H-pyrido[2,3-b] [1,4]benzodiazepine-6-one, methoctramine, and hexahydrosiladifenidol indicated clearly that different muscarinic receptors were involved (M1 and probably M3, respectively). The differential localization of distinct muscarinic receptor subtypes on terminals releasing the major inhibitory and excitatory transmitters of the brain could be exploited therapeutically in some movement disorders and Alzheimer disease.

Original languageEnglish (US)
Pages (from-to)2608-2611
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number6
StatePublished - 1991

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Aminobutyrates
Excitatory Amino Acids
Muscarinic Receptors
Dissociative Disorders
Muscarine
Pirenzepine
Synaptic Potentials
Nucleus Accumbens
Movement Disorders
Brain
Amygdala
Acetylcholine
Alzheimer Disease
Neurons
methoctramine
hexahydrosiladifenidol
otenzepad
In Vitro Techniques

Keywords

  • Amygdala
  • Electrophysiology
  • M and M receptors
  • Presynaptic inhibition
  • Striatum

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Distinct muscarinic receptors inhibit release of γ-aminobutyric acid and excitatory amino acids in mammalian brain. / Sugita, S.; Uchimura, N.; Jiang, Zhi-Gen; North, R. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 6, 1991, p. 2608-2611.

Research output: Contribution to journalArticle

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