Distinct modes of dopamine and GABA release in a dual transmitter neuron

Maria Borisovska, AeSoon L. Bensen, Gene Chong, Gary Westbrook

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We now know of a surprising number of cases where single neurons contain multiple neurotransmitters. Neurons that contain a fastacting neurotransmitter, such as glutamate or GABA, and a modulatory transmitter, such as dopamine, are a particularly interesting case because they presumably serve dual signaling functions. The olfactory bulb contains a large population of GABA- and dopaminecontaining neurons that have been implicated in normal olfaction as well as in Parkinson's disease. Yet, they have been classified as nonexocytotic catecholamine neurons because of the apparent lack of vesicular monoamine transporters. Thus, we examined how dopamine is stored and released from tyrosine hydroxylase-positive GFP (TH+-GFP) mouse periglomerular neurons in vitro. TH+ cells expressed both VMAT2 (vesicular monoamine transporter 2) and VGAT (vesicular GABA transporter), consistent with vesicular storage of both dopamine and GABA. Carbon fiber amperometry revealed that release of dopamine was quantal and calcium-dependent, but quantal size was much less than expected for large dense core vesicles, suggesting that release originated from small clear vesicles identified by electron microscopy. A single action potential in a TH+ neuron evoked a brief GABA-mediated synaptic current, whereas evoked dopamine release was asynchronous, lasting for tens of seconds. Our data suggest that dopamine and GABA serve temporally distinct roles in these dual transmitter neurons.

Original languageEnglish (US)
Pages (from-to)1790-1796
Number of pages7
JournalJournal of Neuroscience
Volume33
Issue number5
DOIs
StatePublished - Jan 30 2013

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gamma-Aminobutyric Acid
Dopamine
Neurons
Vesicular Monoamine Transport Proteins
Neurotransmitter Agents
GABAergic Neurons
Smell
Olfactory Bulb
Secretory Vesicles
Tyrosine 3-Monooxygenase
Action Potentials
Catecholamines
Parkinson Disease
Glutamic Acid
Electron Microscopy
Calcium
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct modes of dopamine and GABA release in a dual transmitter neuron. / Borisovska, Maria; Bensen, AeSoon L.; Chong, Gene; Westbrook, Gary.

In: Journal of Neuroscience, Vol. 33, No. 5, 30.01.2013, p. 1790-1796.

Research output: Contribution to journalArticle

Borisovska, Maria ; Bensen, AeSoon L. ; Chong, Gene ; Westbrook, Gary. / Distinct modes of dopamine and GABA release in a dual transmitter neuron. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 5. pp. 1790-1796.
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