Distinct functions of α-spectrin and β-spectrin during axonal pathfinding

Jörn Hülsmeier, Jan Pielage, Christof Rickert, Gerd M. Technau, Christian Klämbt, Wilm Tobias Stork

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cell-shape changes during development require a precise coupling of the cytoskeleton with proteins situated in the plasma membrane. Important elements controlling the shape of cells are the Spectrin proteins that are expressed as a subcortical cytoskeletal meshwork linking specific membrane receptors with F-actin fibers. Here, we demonstrate that Drosophila karussell mutations affect β-spectrin and lead to distinct axonal patterning defects in the embryonic CNS. karussell mutants display a slit-sensitive axonal phenotype characterized by axonal looping in stage-13 embryos. Further analyses of individual, labeled neuroblast lineages revealed abnormally structured growth cones in these animals. Cell-type-specific rescue experiments demonstrate that β-Spectrin is required autonomously and non-autonomously in cortical neurons to allow normal axonal patterning. Within the cell, β-Spectrin is associated with α-Spectrin. We show that expression of the two genes is tightly regulated by post-translational mechanisms. Loss of β-Spectrin significantly reduces levels of neuronal α-Spectrin expression, whereas gain of β-Spectrin leads to an increase in α-Spectrin protein expression. Because the loss of α-spectrin does not result in an embryonic nervous system phenotype, β-Spectrin appears to act at least partially independent of α-Spectrin to control axonal patterning.

Original languageEnglish (US)
Pages (from-to)713-722
Number of pages10
JournalDevelopment
Volume134
Issue number4
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Spectrin
Cell Shape
Axon Guidance
Phenotype
Growth Cones
Proteins
Cytoskeleton
Nervous System
Drosophila
Actins
Embryonic Structures

Keywords

  • Drosophila
  • Growth cone
  • Nervous system
  • Spectrin

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Hülsmeier, J., Pielage, J., Rickert, C., Technau, G. M., Klämbt, C., & Stork, W. T. (2007). Distinct functions of α-spectrin and β-spectrin during axonal pathfinding. Development, 134(4), 713-722. https://doi.org/10.1242/dev.02758

Distinct functions of α-spectrin and β-spectrin during axonal pathfinding. / Hülsmeier, Jörn; Pielage, Jan; Rickert, Christof; Technau, Gerd M.; Klämbt, Christian; Stork, Wilm Tobias.

In: Development, Vol. 134, No. 4, 01.02.2007, p. 713-722.

Research output: Contribution to journalArticle

Hülsmeier, J, Pielage, J, Rickert, C, Technau, GM, Klämbt, C & Stork, WT 2007, 'Distinct functions of α-spectrin and β-spectrin during axonal pathfinding', Development, vol. 134, no. 4, pp. 713-722. https://doi.org/10.1242/dev.02758
Hülsmeier J, Pielage J, Rickert C, Technau GM, Klämbt C, Stork WT. Distinct functions of α-spectrin and β-spectrin during axonal pathfinding. Development. 2007 Feb 1;134(4):713-722. https://doi.org/10.1242/dev.02758
Hülsmeier, Jörn ; Pielage, Jan ; Rickert, Christof ; Technau, Gerd M. ; Klämbt, Christian ; Stork, Wilm Tobias. / Distinct functions of α-spectrin and β-spectrin during axonal pathfinding. In: Development. 2007 ; Vol. 134, No. 4. pp. 713-722.
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