TY - JOUR
T1 - Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity
AU - Spinelli, Kateri J.
AU - Klimek, John E.
AU - Wilmarth, Phillip A.
AU - Shin, Jung Bum
AU - Choi, Dongseok
AU - David, Larry L.
AU - Gillespie, Peter G.
PY - 2012/1/31
Y1 - 2012/1/31
N2 - Measuring the abundance of many proteins over a broad dynamic range requires accurate quantitation. We show empirically that, in MS experiments, relative quantitation using summed dissociationproduct ion-current intensities is accurate, albeit variable from protein to protein, and outperforms spectral counting. By applying intensities to quantify proteins in two complex but related tissues, chick auditory and vestibular sensory epithelia, we find that glycolytic enzymes are enriched threefold in auditory epithelia, whereas enzymes responsible for oxidative phosphorylation are increased at least fourfold in vestibular epithelia. This striking difference in relative use of the two ATP-production pathways likely reflects the isolation of the auditory epithelium from its blood supply, necessary to prevent heartbeat-induced mechanical disruptions. The global view of protein expression afforded by label-free quantitation with awide dynamic range revealsmolecular specialization at a tissue or cellular level.
AB - Measuring the abundance of many proteins over a broad dynamic range requires accurate quantitation. We show empirically that, in MS experiments, relative quantitation using summed dissociationproduct ion-current intensities is accurate, albeit variable from protein to protein, and outperforms spectral counting. By applying intensities to quantify proteins in two complex but related tissues, chick auditory and vestibular sensory epithelia, we find that glycolytic enzymes are enriched threefold in auditory epithelia, whereas enzymes responsible for oxidative phosphorylation are increased at least fourfold in vestibular epithelia. This striking difference in relative use of the two ATP-production pathways likely reflects the isolation of the auditory epithelium from its blood supply, necessary to prevent heartbeat-induced mechanical disruptions. The global view of protein expression afforded by label-free quantitation with awide dynamic range revealsmolecular specialization at a tissue or cellular level.
UR - http://www.scopus.com/inward/record.url?scp=84857136904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857136904&partnerID=8YFLogxK
U2 - 10.1073/pnas.1115866109
DO - 10.1073/pnas.1115866109
M3 - Article
C2 - 22307652
AN - SCOPUS:84857136904
SN - 0027-8424
VL - 109
SP - E268-E277
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -