Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity

Kateri J. Spinelli, John E. Klimek, Phillip A. Wilmarth, Jung Bum Shin, Dongseok Choi, Larry L. David, Peter G. Gillespie

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Measuring the abundance of many proteins over a broad dynamic range requires accurate quantitation. We show empirically that, in MS experiments, relative quantitation using summed dissociationproduct ion-current intensities is accurate, albeit variable from protein to protein, and outperforms spectral counting. By applying intensities to quantify proteins in two complex but related tissues, chick auditory and vestibular sensory epithelia, we find that glycolytic enzymes are enriched threefold in auditory epithelia, whereas enzymes responsible for oxidative phosphorylation are increased at least fourfold in vestibular epithelia. This striking difference in relative use of the two ATP-production pathways likely reflects the isolation of the auditory epithelium from its blood supply, necessary to prevent heartbeat-induced mechanical disruptions. The global view of protein expression afforded by label-free quantitation with awide dynamic range revealsmolecular specialization at a tissue or cellular level.

Original languageEnglish (US)
Pages (from-to)E268-E277
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number5
DOIs
StatePublished - Jan 31 2012

ASJC Scopus subject areas

  • General

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