Distinct effects of interferon-γ and MHC class I surface antigen levels on resistance of the K562 tumor cell line to natural killer-mediated lysis

Richard Maziarz, Steven J. Mentzer, Steven J. Burakoff, Douglas V. Faller

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Various investigators have examined the relationship between tumor cell susceptibility to natural killer (NK) cell lysis and the expression of HLA class I antigens on the tumor cell. There is controversy as to whether or not an inverse relationship exists, and if so, the basis of the relationship between these two phenomena remains undefined. To address these questions, the genomic clones for two HLA antigens were transfected into the erythroleukemia cell line K562, a cell line that is used as the standard to assess human NK and major histocompatibility complex (MHC) nonrestricted cytolysis. Susceptibility to NK lysis was not affected by the de novo expression of HLA antigens on the K562 after DNA mediated gene transfer. Interferon-γ (IFN-γ) treatment of K562 induced levels of MHC class I antigen surface expression comparable to those found on the transfected cells; however, the IFN-γ-treated cells were resistant to NK lysis. When very high levels of surface HLA antigens were induced on the transfectants, a potential effect of class I MHC expression on K562 lysis could be discerned that was distinct from the resistance to NK lysis induced by IFN-γ treatment.

Original languageEnglish (US)
Pages (from-to)329-338
Number of pages10
JournalCellular Immunology
Volume130
Issue number2
DOIs
StatePublished - Oct 15 1990
Externally publishedYes

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Histocompatibility Antigens Class I
K562 Cells
Surface Antigens
HLA Antigens
Tumor Cell Line
Major Histocompatibility Complex
Interferons
Cell Line
Leukemia, Erythroblastic, Acute
Innate Immunity
Natural Killer Cells
Neoplasms
Clone Cells
Research Personnel
DNA
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Distinct effects of interferon-γ and MHC class I surface antigen levels on resistance of the K562 tumor cell line to natural killer-mediated lysis. / Maziarz, Richard; Mentzer, Steven J.; Burakoff, Steven J.; Faller, Douglas V.

In: Cellular Immunology, Vol. 130, No. 2, 15.10.1990, p. 329-338.

Research output: Contribution to journalArticle

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