Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity

Andrea A. Itano, Stephen J. McSorley, R. Lee Reinhardt, Benjamin (Ben) Ehst, Elizabeth Ingulli, Alexander Y. Rudensky, Marc K. Jenkins

Research output: Contribution to journalArticle

535 Citations (Scopus)

Abstract

Peptide:MHC II complexes derived from a fluorescent antigen were detected in vivo to identify the cells that present subcutaneously injected antigen to CD4 T cells. Skin-derived dendritic cells (DCs) that acquired the antigen while in the draining lymph nodes were the first cells to display peptide:MHC II complexes. Presentation by these cells induced CD69, IL-2 production, and maximal proliferation by the T cells. Later, DCs displaying peptide:MHC II complexes migrated from the injection site via a G protein-dependent mechanism. Presentation by these migrants sustained expression of the IL-2 receptor and promoted delayed type hypersensitivity. Therefore, presentation of peptide:MHC II complexes derived from a subcutaneous antigen occurs in two temporally distinct waves with different functional consequences.

Original languageEnglish (US)
Pages (from-to)47-57
Number of pages11
JournalImmunity
Volume19
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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CD4 Antigens
Cellular Immunity
Dendritic Cells
T-Lymphocytes
Peptides
Antigens
Population
Langerhans Cells
Interleukin-2 Receptors
Delayed Hypersensitivity
GTP-Binding Proteins
Interleukin-2
Lymph Nodes
Injections

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Itano, A. A., McSorley, S. J., Reinhardt, R. L., Ehst, B. B., Ingulli, E., Rudensky, A. Y., & Jenkins, M. K. (2003). Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity. Immunity, 19(1), 47-57. https://doi.org/10.1016/S1074-7613(03)00175-4

Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity. / Itano, Andrea A.; McSorley, Stephen J.; Reinhardt, R. Lee; Ehst, Benjamin (Ben); Ingulli, Elizabeth; Rudensky, Alexander Y.; Jenkins, Marc K.

In: Immunity, Vol. 19, No. 1, 01.07.2003, p. 47-57.

Research output: Contribution to journalArticle

Itano, AA, McSorley, SJ, Reinhardt, RL, Ehst, BB, Ingulli, E, Rudensky, AY & Jenkins, MK 2003, 'Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity', Immunity, vol. 19, no. 1, pp. 47-57. https://doi.org/10.1016/S1074-7613(03)00175-4
Itano, Andrea A. ; McSorley, Stephen J. ; Reinhardt, R. Lee ; Ehst, Benjamin (Ben) ; Ingulli, Elizabeth ; Rudensky, Alexander Y. ; Jenkins, Marc K. / Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity. In: Immunity. 2003 ; Vol. 19, No. 1. pp. 47-57.
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