Distinct Ca2+ sources in dendritic spines of hippocampal CA1 Neurons Couple to SK and Kv4 Channels

Kang Wang, Mike T. Lin, John Adelman, James Maylie

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Small conductance Ca2+-activated K+ (SK) channels and voltage-gated A-type Kv4 channels shape dendritic excitatory postsynaptic potentials (EPSPs) in hippocampal CA1 pyramidal neurons. Synaptically evoked Ca2+ influx through N-methyl-D-aspartate receptors (NMDARs) activates spine SK channels, reducing EPSPs and the associated spine head Ca2+ transient. However, results using glutamate uncaging implicated Ca2+ influx through SNX-482-sensitive (SNX-sensitive) Cav2.3 (R-type) Ca2+ channels as the Ca2+ source for SK channel activation. The present findings show that, using Schaffer collateral stimulation, the effects of SNX and apamin are not mutually exclusive and SNX increases EPSPs independent of SK channel activity. Dialysis with 1,2-bis(o-aminophenoxy)ethane-N'N'N'-tetraacetic acid (BAPTA), application of 4-Aminopyridine (4-AP), expression of a Kv4.2 dominant negative subunit, and dialysis with a KChIPs antibody occluded the SNX-induced increase of EPSPs. The results suggest two distinct Ca2+ signaling pathways within dendritic spines that link Ca2+ influx through NMDARs to SK channels and Ca2+ influx through R-type Ca2+ channels to Kv4.2-containing channels.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalNeuron
Volume81
Issue number2
DOIs
StatePublished - Jan 22 2014

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Dendritic Spines
Excitatory Postsynaptic Potentials
Neurons
N-Methyl-D-Aspartate Receptors
Dialysis
Spine
Apamin
Calcium-Activated Potassium Channels
4-Aminopyridine
Ethane
Pyramidal Cells
Glutamic Acid
Hippocampus
Acids
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct Ca2+ sources in dendritic spines of hippocampal CA1 Neurons Couple to SK and Kv4 Channels. / Wang, Kang; Lin, Mike T.; Adelman, John; Maylie, James.

In: Neuron, Vol. 81, No. 2, 22.01.2014, p. 379-387.

Research output: Contribution to journalArticle

Wang, Kang ; Lin, Mike T. ; Adelman, John ; Maylie, James. / Distinct Ca2+ sources in dendritic spines of hippocampal CA1 Neurons Couple to SK and Kv4 Channels. In: Neuron. 2014 ; Vol. 81, No. 2. pp. 379-387.
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