Dissociation of 68,000 M(r) heat shock protein synthesis from thermotolerance expression in rat fibroblasts

R. B. Widelitz, B. E. Magun, E. W. Gerner

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Rat embryonic fibroblasts growing exponentially at either 35, 37, or 39°C were exposed to 42°C for times up to 6 hr. Cell survival was unaffected by this heat shock in cultures growing at 39°C but survival was decreased in a temperature dependent manner in cells growing at 37 or 35°C. Exposure to 42°C of cells previously adapted to 35 or 37°C resulted in the induction of heat shock proteins (hsps) with apparent molecular weights of 68,000 (hsp 68), 70,000 (hsp 70), and 89,000 (hsp 89); cells previously adapted to 39°C expressed all hsps except hsp 68. Inasmuch as the synthesis of certain hsps may function to protect cells from thermal damage, these data indicate that hsp 68 may not be required for this adaptation-related thermotolerant survival response. Hsp 68 may only be expressed in cells destined to die.

Original languageEnglish (US)
Pages (from-to)433-437
Number of pages5
JournalRadiation Research
Volume99
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

protein synthesis
fibroblasts
Heat-Shock Proteins
heat tolerance
heat shock proteins
rats
Fibroblasts
shock
dissociation
heat
proteins
cells
Hot Temperature
HSP70 Heat-Shock Proteins
Shock
Cell Survival
Molecular Weight
cell viability
heat stress
Thermotolerance

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Dissociation of 68,000 M(r) heat shock protein synthesis from thermotolerance expression in rat fibroblasts. / Widelitz, R. B.; Magun, B. E.; Gerner, E. W.

In: Radiation Research, Vol. 99, No. 2, 1984, p. 433-437.

Research output: Contribution to journalArticle

Widelitz, R. B. ; Magun, B. E. ; Gerner, E. W. / Dissociation of 68,000 M(r) heat shock protein synthesis from thermotolerance expression in rat fibroblasts. In: Radiation Research. 1984 ; Vol. 99, No. 2. pp. 433-437.
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