Dissection of corticotropin-releasing factor system involvement in locomotor sensitivity to methamphetamine

W. J. Giardino, R. Pastor, A. M J Anacker, E. Spangler, D. M. Cote, J. Li, Mary Stenzel-Poore, Tamara Phillips, Andrey Ryabinin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sensitivity to the euphoric and locomotor-activating effects of drugs of abuse may contribute to risk for excessive use and addiction. Repeated administration of psychostimulants such as methamphetamine (MA) can result in neuroadaptive consequences that manifest behaviorally as a progressive escalation of locomotor activation, termed psychomotor sensitization. The present studies addressed the involvement of specific components of the corticotropin-releasing factor (CRF) system in locomotor activation and psychomotor sensitization induced by MA (1, 2 mg/kg) by utilizing pharmacological approaches, as well as a series of genetic knockout (KO) mice, each deficient for a single component of the CRF system: CRF-R1, CRF-R2, CRF, or the CRF-related peptide Urocortin 1 (Ucn1). CRF-R1 KO mice did not differ from wild-type mice in sensitization to MA, and pharmacological blockade of CRF-R1 with CP-154,526 (15, 30 mg/kg) in DBA/2J mice did not selectively attenuate either the acquisition or expression of MA-induced sensitization. Deletion of either of the endogenous ligands of CRF-R1 (CRF, Ucn1) either enhanced or had no effect on MA-induced sensitization, providing further evidence against a role for CRF-R1 signaling. Interestingly, deletion of CRF-R2 attenuated MA-induced locomotor activation, elucidating a novel contribution of the CRF system to MA sensitivity, and suggesting the participation of the endogenous urocortin peptides Ucn2 and Ucn3. Immunohistochemistry for Fos was used to visualize neural activation underlying CRF-R2-dependent sensitivity to MA, identifying the basolateral and central nuclei of the amygdala as neural substrates involved in this response. Our results support further examination of CRF-R2 involvement in neural processes associated with MA addiction.

Original languageEnglish (US)
Pages (from-to)78-89
Number of pages12
JournalGenes, Brain and Behavior
Volume10
Issue number1
DOIs
StatePublished - Feb 2011

Fingerprint

Methamphetamine
Corticotropin-Releasing Hormone
Dissection
Urocortins
Knockout Mice
Pharmacology
Inbred DBA Mouse
Peptides
Street Drugs

Keywords

  • Addiction
  • Amphetamine
  • Amygdala
  • Corticotropin-releasing hormone
  • Urocortin

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Genetics
  • Neurology

Cite this

Dissection of corticotropin-releasing factor system involvement in locomotor sensitivity to methamphetamine. / Giardino, W. J.; Pastor, R.; Anacker, A. M J; Spangler, E.; Cote, D. M.; Li, J.; Stenzel-Poore, Mary; Phillips, Tamara; Ryabinin, Andrey.

In: Genes, Brain and Behavior, Vol. 10, No. 1, 02.2011, p. 78-89.

Research output: Contribution to journalArticle

Giardino, W. J. ; Pastor, R. ; Anacker, A. M J ; Spangler, E. ; Cote, D. M. ; Li, J. ; Stenzel-Poore, Mary ; Phillips, Tamara ; Ryabinin, Andrey. / Dissection of corticotropin-releasing factor system involvement in locomotor sensitivity to methamphetamine. In: Genes, Brain and Behavior. 2011 ; Vol. 10, No. 1. pp. 78-89.
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