Disruption of an Active Site Hydrogen Bond Converts Human Heme Oxygenase-1 into a Peroxidase

Luke Koenigs Lightning, Hong Wei Huang, Pierre Moenne-Loccoz, Thomas M. Loehr, David J. Schuller, Thomas L. Poulos, Paul R. Ortiz De Montellano

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The crystal structure of heme oxygenase-1 suggests that Asp-140 may participate in a hydrogen bonding network involving ligands coordinated to the heme iron atom. To examine this possibility, Asp-140 was mutated to an alanine, phenylalanine, histidine, leucine, or asparagine, and the properties of the purified proteins were investigated. UV-visible and resonance Raman spectroscopy indicate that the distal water ligand is lost from the iron in all the mutants except, to some extent, the D140N mutant. In the D140H mutant, the distal water ligand is replaced by the new His-140 as the sixth iron ligand, giving a bis-histidine complex. The D140A, D140H, and D140N mutants retain a trace (

Original languageEnglish (US)
Pages (from-to)10612-10619
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number14
DOIs
StatePublished - Apr 6 2001

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Heme Oxygenase-1
Peroxidase
Hydrogen
Catalytic Domain
Hydrogen bonds
Ligands
Iron
Histidine
Raman Spectrum Analysis
Water
Asparagine
Hydrogen Bonding
Phenylalanine
Heme
Leucine
Alanine
Raman spectroscopy
Crystal structure
Atoms
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lightning, L. K., Huang, H. W., Moenne-Loccoz, P., Loehr, T. M., Schuller, D. J., Poulos, T. L., & Ortiz De Montellano, P. R. (2001). Disruption of an Active Site Hydrogen Bond Converts Human Heme Oxygenase-1 into a Peroxidase. Journal of Biological Chemistry, 276(14), 10612-10619. https://doi.org/10.1074/jbc.M010349200

Disruption of an Active Site Hydrogen Bond Converts Human Heme Oxygenase-1 into a Peroxidase. / Lightning, Luke Koenigs; Huang, Hong Wei; Moenne-Loccoz, Pierre; Loehr, Thomas M.; Schuller, David J.; Poulos, Thomas L.; Ortiz De Montellano, Paul R.

In: Journal of Biological Chemistry, Vol. 276, No. 14, 06.04.2001, p. 10612-10619.

Research output: Contribution to journalArticle

Lightning, LK, Huang, HW, Moenne-Loccoz, P, Loehr, TM, Schuller, DJ, Poulos, TL & Ortiz De Montellano, PR 2001, 'Disruption of an Active Site Hydrogen Bond Converts Human Heme Oxygenase-1 into a Peroxidase', Journal of Biological Chemistry, vol. 276, no. 14, pp. 10612-10619. https://doi.org/10.1074/jbc.M010349200
Lightning, Luke Koenigs ; Huang, Hong Wei ; Moenne-Loccoz, Pierre ; Loehr, Thomas M. ; Schuller, David J. ; Poulos, Thomas L. ; Ortiz De Montellano, Paul R. / Disruption of an Active Site Hydrogen Bond Converts Human Heme Oxygenase-1 into a Peroxidase. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 14. pp. 10612-10619.
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