Disinfection capacity of PuriLens contact lens cleaning unit against acanthamoeba

Thomas Hwang, Young Hyon Joon, Kyung Song Jae, Victor E. Reviglio, Harry T. Spahr, Terrence P. O'Brien

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose. The PuriLens contact lens system is indicated for cleaning and disinfection of soft (hydrophilic) contact lenses by means of subsonic agitation to remove lens deposits and microorganisms, and ultraviolet irradiation of the storage solution for disinfection. The capacity of the PuriLens system to disinfect storage solutions contaminated with known concentrations of Staphylococcus aureus, Pseudomonas aeruginosa, and Acanthamoeba species was evaluated. Methods. An in vitro assessment of the antibacterial and antiparasitic efficacy of the PuriLens system was performed. Separated batches of the storage solution for the cleansing system were contaminated with stock strains of S. aureus and P. aeruginosa. A comparison of the microbiologic content was made between the solution before and after the cycle. Results. The PuriLens system effectively eradicated S. aureus and P. aeruginosa organisms after a 15-minute cycle. However, viable cysts of acanthamoeba were recovered in the solution after the 15-minute cycle. Conclusions. The PuriLens system is highly efficient in protecting against contamination with common bacterial ocular pathogens. Acanthamoeba cysts, however, can survive in the solution or contact lens bath undergoing integrated subsonic debridement and indirect ultraviolet light disinfection. Use of chemical disinfecting solutions that contain agents such as chlorhexidine or other cationic antiseptics may be advisable in conjunction with use of the PuriLens device, especially in high-risk settings.

Original languageEnglish (US)
Pages (from-to)42-43
Number of pages2
JournalEye and Contact Lens
Volume30
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Acanthamoeba
Disinfection
Contact Lenses
Pseudomonas aeruginosa
Hydrophilic Contact Lens
Staphylococcus aureus
Cysts
Contact Lens Solutions
Antiparasitic Agents
Local Anti-Infective Agents
Chlorhexidine
Debridement
Ultraviolet Rays
Baths
Lenses
Equipment and Supplies

Keywords

  • Acanthamoeba keratitis
  • Contact lens
  • Disinfection
  • PuriLens

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Disinfection capacity of PuriLens contact lens cleaning unit against acanthamoeba. / Hwang, Thomas; Joon, Young Hyon; Jae, Kyung Song; Reviglio, Victor E.; Spahr, Harry T.; O'Brien, Terrence P.

In: Eye and Contact Lens, Vol. 30, No. 1, 01.2004, p. 42-43.

Research output: Contribution to journalArticle

Hwang, Thomas ; Joon, Young Hyon ; Jae, Kyung Song ; Reviglio, Victor E. ; Spahr, Harry T. ; O'Brien, Terrence P. / Disinfection capacity of PuriLens contact lens cleaning unit against acanthamoeba. In: Eye and Contact Lens. 2004 ; Vol. 30, No. 1. pp. 42-43.
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abstract = "Purpose. The PuriLens contact lens system is indicated for cleaning and disinfection of soft (hydrophilic) contact lenses by means of subsonic agitation to remove lens deposits and microorganisms, and ultraviolet irradiation of the storage solution for disinfection. The capacity of the PuriLens system to disinfect storage solutions contaminated with known concentrations of Staphylococcus aureus, Pseudomonas aeruginosa, and Acanthamoeba species was evaluated. Methods. An in vitro assessment of the antibacterial and antiparasitic efficacy of the PuriLens system was performed. Separated batches of the storage solution for the cleansing system were contaminated with stock strains of S. aureus and P. aeruginosa. A comparison of the microbiologic content was made between the solution before and after the cycle. Results. The PuriLens system effectively eradicated S. aureus and P. aeruginosa organisms after a 15-minute cycle. However, viable cysts of acanthamoeba were recovered in the solution after the 15-minute cycle. Conclusions. The PuriLens system is highly efficient in protecting against contamination with common bacterial ocular pathogens. Acanthamoeba cysts, however, can survive in the solution or contact lens bath undergoing integrated subsonic debridement and indirect ultraviolet light disinfection. Use of chemical disinfecting solutions that contain agents such as chlorhexidine or other cationic antiseptics may be advisable in conjunction with use of the PuriLens device, especially in high-risk settings.",
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