Discrimination of intra- and extracellular 23Na+ signals in yeast cell suspensions using longitudinal magnetic resonance relaxography

Yajie Zhang, Marie Poirer-Quinot, Charles Jr Springer, James A. Balschi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study tested the ability of MR relaxography (MRR) to discriminate intra- (Nai+) and extracellular (Nae+) 23Na+ signals using their longitudinal relaxation time constant (T1) values. Na +-loaded yeast cell (Saccharomyces cerevisiae) suspensions were investigated. Two types of compartmental 23Na+ T 1 differences were examined: a selective Nae+ T1 decrease induced by an extracellular relaxation reagent (RRe), GdDOTP 5-; and, an intrinsic T1 difference. Parallel studies using the established method of 23Na MRS with an extracellular shift reagent (SRe), TmDOTP5-, were used to validate the MRR measurements. With 12.8 mM RRe, the 23Nae+ T1 was 2.4 ms and the 23Nai+ T1 was 9.5 ms (9.4T, 24 °C). The Na+ amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RRe or by MRS/SR e. Without RRe, the Na+-loaded yeast cell suspension 23Na MR signal exhibited two T1 values, 9.1 (±0.3) ms and 32.7 (±2.3) ms, assigned to 23Nai+ and 23Nae+, respectively. The Nai+ content measured was lower, 0.88 (±0.06); while Nae+ was higher, 1.43 (±0.12) compared with MRS/SRe measures on the same samples. However, the measured efflux rate constant was identical. T1 MRR potentially may be used for Nai+ determination in vivo and Na+ flux measurements; with RRe for animal studies and without RRe for humans.

Original languageEnglish (US)
Pages (from-to)28-37
Number of pages10
JournalJournal of Magnetic Resonance
Volume205
Issue number1
DOIs
StatePublished - Jul 2010

Fingerprint

yeast
Magnetic resonance
Yeast
reagents
magnetic resonance
discrimination
Suspensions
Magnetic Resonance Spectroscopy
Yeasts
Cells
efflux
Rate constants
saccharomyces
shift
Relaxation time
time constant
Saccharomyces cerevisiae
animals
Animals
relaxation time

Keywords

  • Na MR
  • Intracellular Na
  • Relaxation reagent
  • T relaxography

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Medicine(all)

Cite this

Discrimination of intra- and extracellular 23Na+ signals in yeast cell suspensions using longitudinal magnetic resonance relaxography. / Zhang, Yajie; Poirer-Quinot, Marie; Springer, Charles Jr; Balschi, James A.

In: Journal of Magnetic Resonance, Vol. 205, No. 1, 07.2010, p. 28-37.

Research output: Contribution to journalArticle

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abstract = "This study tested the ability of MR relaxography (MRR) to discriminate intra- (Nai+) and extracellular (Nae+) 23Na+ signals using their longitudinal relaxation time constant (T1) values. Na +-loaded yeast cell (Saccharomyces cerevisiae) suspensions were investigated. Two types of compartmental 23Na+ T 1 differences were examined: a selective Nae+ T1 decrease induced by an extracellular relaxation reagent (RRe), GdDOTP 5-; and, an intrinsic T1 difference. Parallel studies using the established method of 23Na MRS with an extracellular shift reagent (SRe), TmDOTP5-, were used to validate the MRR measurements. With 12.8 mM RRe, the 23Nae+ T1 was 2.4 ms and the 23Nai+ T1 was 9.5 ms (9.4T, 24 °C). The Na+ amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RRe or by MRS/SR e. Without RRe, the Na+-loaded yeast cell suspension 23Na MR signal exhibited two T1 values, 9.1 (±0.3) ms and 32.7 (±2.3) ms, assigned to 23Nai+ and 23Nae+, respectively. The Nai+ content measured was lower, 0.88 (±0.06); while Nae+ was higher, 1.43 (±0.12) compared with MRS/SRe measures on the same samples. However, the measured efflux rate constant was identical. T1 MRR potentially may be used for Nai+ determination in vivo and Na+ flux measurements; with RRe for animal studies and without RRe for humans.",
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