Discrimination of angiotensin II receptor subtype distribution in the rat brain using non-peptidic receptor antagonists

Brian P. Rowe; Kevin L. Grove; David L. Saylor; Robert C. Speth

doi:10.1016/0167-0115(91)90014-8

Discrimination of angiotensin II receptor subtype distribution in the rat brain using non-peptidic receptor antagonists

Brian P. Rowe, Kevin L. Grove, David L. Saylor, Robert C. Speth

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

The non-peptidic angiotensin II receptor subtype selective antagonists, DuP 753 and PD123177, were used to characterize angiotensin II receptor binding sites in the rat brain. Competitive receptor autoradiography with ¹²⁵I-Sar¹-Ile⁸ angiotensin II defined a regional distribution of binding sites that were sensitive to either DuP 753 (designated AII_α subtype) or PD123177 (designated AII_β subtype). Whereas most brain nuclei could be assigned to a category containing a predominant subtype, a multiple receptor subtype analysis indicated that some regions are homogeneous, while others contain a mixture of both AII_α and AII_β subtypes.

Original language	English (US)
Pages (from-to)	45-53
Number of pages	9
Journal	Regulatory Peptides
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/0167-0115(91)90014-8
State	Published - Mar 26 1991
Externally published	Yes

Keywords

Angiotensin II receptor
DuP 753
PD123177
Rat brain
Receptor autoradiography
Receptor subtype

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/0167-0115(91)90014-8

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title = "Discrimination of angiotensin II receptor subtype distribution in the rat brain using non-peptidic receptor antagonists",

abstract = "The non-peptidic angiotensin II receptor subtype selective antagonists, DuP 753 and PD123177, were used to characterize angiotensin II receptor binding sites in the rat brain. Competitive receptor autoradiography with 125I-Sar1-Ile8 angiotensin II defined a regional distribution of binding sites that were sensitive to either DuP 753 (designated AIIα subtype) or PD123177 (designated AIIβ subtype). Whereas most brain nuclei could be assigned to a category containing a predominant subtype, a multiple receptor subtype analysis indicated that some regions are homogeneous, while others contain a mixture of both AIIα and AIIβ subtypes.",

keywords = "Angiotensin II receptor, DuP 753, PD123177, Rat brain, Receptor autoradiography, Receptor subtype",

author = "Rowe, {Brian P.} and Grove, {Kevin L.} and Saylor, {David L.} and Speth, {Robert C.}",

note = "Funding Information: DuP 753 and PD123177 were generously supplied by Dr. Pieter Timmermans of DuPont. We thank Dr. Michael Woodruff for neuroanatomical assistance, Teresa Lloyd-Saylor for technical assistance and Jean Kintzel and Leisa Gouge for clerical help. Supported by the American Heart Association, Tennessee Affiliate, NIH (NS-24388) and BRSG SO7 RR05465.",

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language = "English (US)",

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T1 - Discrimination of angiotensin II receptor subtype distribution in the rat brain using non-peptidic receptor antagonists

AU - Rowe, Brian P.

AU - Grove, Kevin L.

AU - Saylor, David L.

AU - Speth, Robert C.

N1 - Funding Information: DuP 753 and PD123177 were generously supplied by Dr. Pieter Timmermans of DuPont. We thank Dr. Michael Woodruff for neuroanatomical assistance, Teresa Lloyd-Saylor for technical assistance and Jean Kintzel and Leisa Gouge for clerical help. Supported by the American Heart Association, Tennessee Affiliate, NIH (NS-24388) and BRSG SO7 RR05465.

PY - 1991/3/26

Y1 - 1991/3/26

N2 - The non-peptidic angiotensin II receptor subtype selective antagonists, DuP 753 and PD123177, were used to characterize angiotensin II receptor binding sites in the rat brain. Competitive receptor autoradiography with 125I-Sar1-Ile8 angiotensin II defined a regional distribution of binding sites that were sensitive to either DuP 753 (designated AIIα subtype) or PD123177 (designated AIIβ subtype). Whereas most brain nuclei could be assigned to a category containing a predominant subtype, a multiple receptor subtype analysis indicated that some regions are homogeneous, while others contain a mixture of both AIIα and AIIβ subtypes.

AB - The non-peptidic angiotensin II receptor subtype selective antagonists, DuP 753 and PD123177, were used to characterize angiotensin II receptor binding sites in the rat brain. Competitive receptor autoradiography with 125I-Sar1-Ile8 angiotensin II defined a regional distribution of binding sites that were sensitive to either DuP 753 (designated AIIα subtype) or PD123177 (designated AIIβ subtype). Whereas most brain nuclei could be assigned to a category containing a predominant subtype, a multiple receptor subtype analysis indicated that some regions are homogeneous, while others contain a mixture of both AIIα and AIIβ subtypes.

KW - Angiotensin II receptor

KW - DuP 753

KW - PD123177

KW - Rat brain

KW - Receptor autoradiography

KW - Receptor subtype

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IS - 1

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Discrimination of angiotensin II receptor subtype distribution in the rat brain using non-peptidic receptor antagonists

Abstract

Keywords

ASJC Scopus subject areas

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