Directional optical coherence tomography reveals melanin concentration-dependent scattering properties of retinal pigment epithelium

Ratheesh K. Meleppat, Pengfei Zhang, Myeong Jin Ju, Suman K. Manna, Yifan Jian, Edward N. Pugh, Robert J. Zawadzki

Research output: Contribution to journalArticle

Abstract

Optical coherence tomography (OCT) is a powerful tool in ophthalmology that provides in vivo morphology of the retinal layers and their light scattering properties. The directional (angular) reflectivity of the retinal layers was investigated with focus on the scattering from retinal pigment epithelium (RPE). The directional scattering of the RPE was studied in three mice strains with three distinct melanin concentrations: albino (BALB/c), agouti (129S1/SvlmJ), and strongly pigmented (C57BL/6J). The backscattering signal strength was measured with a directional OCT system in which the pupil entry position of the narrow OCT beam can be varied across the dilated pupil of the eyes of the mice. The directional reflectivity of other retinal melanin-free layers, including the internal and external limiting membranes, and Bruch's membrane (albinos) were also measured and compared between the strains. The intensity of light backscattered from these layers was found highly sensitive to the angle of illumination, whereas the inner/outer segment (IS/OS) junctions showed a reduced sensitivity. The reflections from the RPE are largely insensitive in highly pigmented mice. The differences in directional scattering between strains shows that directionality decreases with an increase in melanin concentrations in RPE, suggesting increasing contribution of Mie scattering by melanosomes.

Original languageEnglish (US)
Article number066011
JournalJournal of biomedical optics
Volume24
Issue number6
DOIs
StatePublished - Jun 30 2019

Fingerprint

Melanin
melanin
Retinal Pigments
epithelium
Optical tomography
Melanins
pigments
Pigments
tomography
Scattering
mice
pupils
scattering
Ophthalmology
ophthalmology
membranes
Membranes
reflectance
Mie scattering
Backscattering

Keywords

  • directional scattering
  • melanin
  • optical coherence tomography
  • retinal imaging
  • retinal pigment epithelium

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Directional optical coherence tomography reveals melanin concentration-dependent scattering properties of retinal pigment epithelium. / Meleppat, Ratheesh K.; Zhang, Pengfei; Ju, Myeong Jin; Manna, Suman K.; Jian, Yifan; Pugh, Edward N.; Zawadzki, Robert J.

In: Journal of biomedical optics, Vol. 24, No. 6, 066011, 30.06.2019.

Research output: Contribution to journalArticle

Meleppat, Ratheesh K. ; Zhang, Pengfei ; Ju, Myeong Jin ; Manna, Suman K. ; Jian, Yifan ; Pugh, Edward N. ; Zawadzki, Robert J. / Directional optical coherence tomography reveals melanin concentration-dependent scattering properties of retinal pigment epithelium. In: Journal of biomedical optics. 2019 ; Vol. 24, No. 6.
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