Abstract
Optical coherence tomography (OCT) is a powerful tool in ophthalmology that provides in vivo morphology of the retinal layers and their light scattering properties. The directional (angular) reflectivity of the retinal layers was investigated with focus on the scattering from retinal pigment epithelium (RPE). The directional scattering of the RPE was studied in three mice strains with three distinct melanin concentrations: albino (BALB/c), agouti (129S1/SvlmJ), and strongly pigmented (C57BL/6J). The backscattering signal strength was measured with a directional OCT system in which the pupil entry position of the narrow OCT beam can be varied across the dilated pupil of the eyes of the mice. The directional reflectivity of other retinal melanin-free layers, including the internal and external limiting membranes, and Bruch's membrane (albinos) were also measured and compared between the strains. The intensity of light backscattered from these layers was found highly sensitive to the angle of illumination, whereas the inner/outer segment (IS/OS) junctions showed a reduced sensitivity. The reflections from the RPE are largely insensitive in highly pigmented mice. The differences in directional scattering between strains shows that directionality decreases with an increase in melanin concentrations in RPE, suggesting increasing contribution of Mie scattering by melanosomes.
| Original language | English (US) |
|---|---|
| Article number | 066011 |
| Journal | Journal of biomedical optics |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 30 2019 |
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Keywords
- directional scattering
- melanin
- optical coherence tomography
- retinal imaging
- retinal pigment epithelium
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
Cite this
Directional optical coherence tomography reveals melanin concentration-dependent scattering properties of retinal pigment epithelium. / Meleppat, Ratheesh K.; Zhang, Pengfei; Ju, Myeong Jin; Manna, Suman K.; Jian, Yifan; Pugh, Edward N.; Zawadzki, Robert J.
In: Journal of biomedical optics, Vol. 24, No. 6, 066011, 30.06.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Directional optical coherence tomography reveals melanin concentration-dependent scattering properties of retinal pigment epithelium
AU - Meleppat, Ratheesh K.
AU - Zhang, Pengfei
AU - Ju, Myeong Jin
AU - Manna, Suman K.
AU - Jian, Yifan
AU - Pugh, Edward N.
AU - Zawadzki, Robert J.
PY - 2019/6/30
Y1 - 2019/6/30
N2 - Optical coherence tomography (OCT) is a powerful tool in ophthalmology that provides in vivo morphology of the retinal layers and their light scattering properties. The directional (angular) reflectivity of the retinal layers was investigated with focus on the scattering from retinal pigment epithelium (RPE). The directional scattering of the RPE was studied in three mice strains with three distinct melanin concentrations: albino (BALB/c), agouti (129S1/SvlmJ), and strongly pigmented (C57BL/6J). The backscattering signal strength was measured with a directional OCT system in which the pupil entry position of the narrow OCT beam can be varied across the dilated pupil of the eyes of the mice. The directional reflectivity of other retinal melanin-free layers, including the internal and external limiting membranes, and Bruch's membrane (albinos) were also measured and compared between the strains. The intensity of light backscattered from these layers was found highly sensitive to the angle of illumination, whereas the inner/outer segment (IS/OS) junctions showed a reduced sensitivity. The reflections from the RPE are largely insensitive in highly pigmented mice. The differences in directional scattering between strains shows that directionality decreases with an increase in melanin concentrations in RPE, suggesting increasing contribution of Mie scattering by melanosomes.
AB - Optical coherence tomography (OCT) is a powerful tool in ophthalmology that provides in vivo morphology of the retinal layers and their light scattering properties. The directional (angular) reflectivity of the retinal layers was investigated with focus on the scattering from retinal pigment epithelium (RPE). The directional scattering of the RPE was studied in three mice strains with three distinct melanin concentrations: albino (BALB/c), agouti (129S1/SvlmJ), and strongly pigmented (C57BL/6J). The backscattering signal strength was measured with a directional OCT system in which the pupil entry position of the narrow OCT beam can be varied across the dilated pupil of the eyes of the mice. The directional reflectivity of other retinal melanin-free layers, including the internal and external limiting membranes, and Bruch's membrane (albinos) were also measured and compared between the strains. The intensity of light backscattered from these layers was found highly sensitive to the angle of illumination, whereas the inner/outer segment (IS/OS) junctions showed a reduced sensitivity. The reflections from the RPE are largely insensitive in highly pigmented mice. The differences in directional scattering between strains shows that directionality decreases with an increase in melanin concentrations in RPE, suggesting increasing contribution of Mie scattering by melanosomes.
KW - directional scattering
KW - melanin
KW - optical coherence tomography
KW - retinal imaging
KW - retinal pigment epithelium
UR - http://www.scopus.com/inward/record.url?scp=85068994316&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068994316&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.24.6.066011
DO - 10.1117/1.JBO.24.6.066011
M3 - Article
C2 - 31254332
AN - SCOPUS:85068994316
VL - 24
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 6
M1 - 066011
ER -