Directed growth of rat hippocampal neurons in microfluidic culture without surface patterning or chemical gradients

Alexander C. Barbati, Cheng Fang, Gary A. Banker, Brian J. Kirby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a microfluidic device that controls rat hippocampal neurons without chemical surface patterning or chemical gradients and is capable of solute delivery to discrete sections of neurons with spatial and temporal resolution. Through the use of analytical expressions and computational models, we select device geometry based upon injection time, ability to confine solutes, and shear stress considerations. Preliminary results show guidance of rat hippocampal neurons within channels, response of mitochondrial transport to toxins, and theoretical solute distributions as a function of device parameters.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages40-42
Number of pages3
StatePublished - Dec 1 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Hippocampal neurons
  • Mitochondrial transport
  • Neural cell culture

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Barbati, A. C., Fang, C., Banker, G. A., & Kirby, B. J. (2010). Directed growth of rat hippocampal neurons in microfluidic culture without surface patterning or chemical gradients. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 40-42). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).