TY - GEN
T1 - Directed growth of rat hippocampal neurons in microfluidic culture without surface patterning or chemical gradients
AU - Barbati, Alexander C.
AU - Fang, Cheng
AU - Banker, Gary A.
AU - Kirby, Brian J.
PY - 2010
Y1 - 2010
N2 - We present a microfluidic device that controls rat hippocampal neurons without chemical surface patterning or chemical gradients and is capable of solute delivery to discrete sections of neurons with spatial and temporal resolution. Through the use of analytical expressions and computational models, we select device geometry based upon injection time, ability to confine solutes, and shear stress considerations. Preliminary results show guidance of rat hippocampal neurons within channels, response of mitochondrial transport to toxins, and theoretical solute distributions as a function of device parameters.
AB - We present a microfluidic device that controls rat hippocampal neurons without chemical surface patterning or chemical gradients and is capable of solute delivery to discrete sections of neurons with spatial and temporal resolution. Through the use of analytical expressions and computational models, we select device geometry based upon injection time, ability to confine solutes, and shear stress considerations. Preliminary results show guidance of rat hippocampal neurons within channels, response of mitochondrial transport to toxins, and theoretical solute distributions as a function of device parameters.
KW - Hippocampal neurons
KW - Mitochondrial transport
KW - Neural cell culture
UR - http://www.scopus.com/inward/record.url?scp=84884323544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884323544&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884323544
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 40
EP - 42
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -