Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian hormone production

Jing Xu, Jon Hennebold, David Seifer

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Objective To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. Design Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly assigned to experimental groups of control, low-dose VD3 (LVD3; 25 pg/mL), and high-dose VD3 (HVD3; 100 pg/mL). Setting National primate research center. Animal(s) Adult, female rhesus macaques (Macaca mulatta). Intervention(s) None. Main Outcome Measure(s) Follicle survival and growth, as well as oocyte size, were assessed. Progesterone (P4), androstenedione (A4), E2, and antimüllerian hormone (AMH) concentrations in culture media were measured. Result(s) Compared with the control group, LVD3 increased preantral follicle survival at week 2 by >66%, while HVD3 increased antral follicle diameters at week 5. Follicles with diameters ≥500 μm at week 5 were categorized as fast-growing follicles. Higher percentages of fast-growing follicles were obtained after HVD3 treatment. Although P4, A4, and E2 production by antral follicles was not altered by VD3, AMH concentrations were 36% higher in the LVD3 group relative to controls at week 5. Oocytes with larger diameters were retrieved from antral follicles developed in both LVD3 and HVD3 groups compared with controls. Conclusion(s) The addition of LVD3 increased preantral follicle survival and maintained AMH production by antral follicles, while HVD3 improved antral follicle growth. VD3 supplement promoted oocyte growth in in vitro–developed follicles. Direct actions of VD3 on the primate follicle appear to be both dose and stage dependent.

    Original languageEnglish (US)
    Pages (from-to)1815-1820.e1
    JournalFertility and Sterility
    Volume106
    Issue number7
    DOIs
    StatePublished - Dec 1 2016

    Fingerprint

    Cholecalciferol
    Macaca mulatta
    Steroids
    Hormones
    Primates
    Oocytes
    Growth
    Control Groups
    Androstenedione
    Progesterone
    Culture Media
    Ovary
    Outcome Assessment (Health Care)
    Antral
    Research

    Keywords

    • antimüllerian hormone
    • follicle culture
    • ovary
    • primate folliculogenesis
    • Vitamin D3

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology

    Cite this

    @article{b6ad65d5d1f049df9b92ba2ee0ccf83d,
    title = "Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antim{\"u}llerian hormone production",
    abstract = "Objective To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. Design Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly assigned to experimental groups of control, low-dose VD3 (LVD3; 25 pg/mL), and high-dose VD3 (HVD3; 100 pg/mL). Setting National primate research center. Animal(s) Adult, female rhesus macaques (Macaca mulatta). Intervention(s) None. Main Outcome Measure(s) Follicle survival and growth, as well as oocyte size, were assessed. Progesterone (P4), androstenedione (A4), E2, and antim{\"u}llerian hormone (AMH) concentrations in culture media were measured. Result(s) Compared with the control group, LVD3 increased preantral follicle survival at week 2 by >66{\%}, while HVD3 increased antral follicle diameters at week 5. Follicles with diameters ≥500 μm at week 5 were categorized as fast-growing follicles. Higher percentages of fast-growing follicles were obtained after HVD3 treatment. Although P4, A4, and E2 production by antral follicles was not altered by VD3, AMH concentrations were 36{\%} higher in the LVD3 group relative to controls at week 5. Oocytes with larger diameters were retrieved from antral follicles developed in both LVD3 and HVD3 groups compared with controls. Conclusion(s) The addition of LVD3 increased preantral follicle survival and maintained AMH production by antral follicles, while HVD3 improved antral follicle growth. VD3 supplement promoted oocyte growth in in vitro–developed follicles. Direct actions of VD3 on the primate follicle appear to be both dose and stage dependent.",
    keywords = "antim{\"u}llerian hormone, follicle culture, ovary, primate folliculogenesis, Vitamin D3",
    author = "Jing Xu and Jon Hennebold and David Seifer",
    year = "2016",
    month = "12",
    day = "1",
    doi = "10.1016/j.fertnstert.2016.08.037",
    language = "English (US)",
    volume = "106",
    pages = "1815--1820.e1",
    journal = "Fertility and Sterility",
    issn = "0015-0282",
    publisher = "Elsevier Inc.",
    number = "7",

    }

    TY - JOUR

    T1 - Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture

    T2 - assessment of follicle survival, growth, steroid, and antimüllerian hormone production

    AU - Xu, Jing

    AU - Hennebold, Jon

    AU - Seifer, David

    PY - 2016/12/1

    Y1 - 2016/12/1

    N2 - Objective To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. Design Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly assigned to experimental groups of control, low-dose VD3 (LVD3; 25 pg/mL), and high-dose VD3 (HVD3; 100 pg/mL). Setting National primate research center. Animal(s) Adult, female rhesus macaques (Macaca mulatta). Intervention(s) None. Main Outcome Measure(s) Follicle survival and growth, as well as oocyte size, were assessed. Progesterone (P4), androstenedione (A4), E2, and antimüllerian hormone (AMH) concentrations in culture media were measured. Result(s) Compared with the control group, LVD3 increased preantral follicle survival at week 2 by >66%, while HVD3 increased antral follicle diameters at week 5. Follicles with diameters ≥500 μm at week 5 were categorized as fast-growing follicles. Higher percentages of fast-growing follicles were obtained after HVD3 treatment. Although P4, A4, and E2 production by antral follicles was not altered by VD3, AMH concentrations were 36% higher in the LVD3 group relative to controls at week 5. Oocytes with larger diameters were retrieved from antral follicles developed in both LVD3 and HVD3 groups compared with controls. Conclusion(s) The addition of LVD3 increased preantral follicle survival and maintained AMH production by antral follicles, while HVD3 improved antral follicle growth. VD3 supplement promoted oocyte growth in in vitro–developed follicles. Direct actions of VD3 on the primate follicle appear to be both dose and stage dependent.

    AB - Objective To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. Design Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly assigned to experimental groups of control, low-dose VD3 (LVD3; 25 pg/mL), and high-dose VD3 (HVD3; 100 pg/mL). Setting National primate research center. Animal(s) Adult, female rhesus macaques (Macaca mulatta). Intervention(s) None. Main Outcome Measure(s) Follicle survival and growth, as well as oocyte size, were assessed. Progesterone (P4), androstenedione (A4), E2, and antimüllerian hormone (AMH) concentrations in culture media were measured. Result(s) Compared with the control group, LVD3 increased preantral follicle survival at week 2 by >66%, while HVD3 increased antral follicle diameters at week 5. Follicles with diameters ≥500 μm at week 5 were categorized as fast-growing follicles. Higher percentages of fast-growing follicles were obtained after HVD3 treatment. Although P4, A4, and E2 production by antral follicles was not altered by VD3, AMH concentrations were 36% higher in the LVD3 group relative to controls at week 5. Oocytes with larger diameters were retrieved from antral follicles developed in both LVD3 and HVD3 groups compared with controls. Conclusion(s) The addition of LVD3 increased preantral follicle survival and maintained AMH production by antral follicles, while HVD3 improved antral follicle growth. VD3 supplement promoted oocyte growth in in vitro–developed follicles. Direct actions of VD3 on the primate follicle appear to be both dose and stage dependent.

    KW - antimüllerian hormone

    KW - follicle culture

    KW - ovary

    KW - primate folliculogenesis

    KW - Vitamin D3

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