@article{d2da573fd0a24e8c9397b81fbcf88355,
title = "Direct High-resolution Nuclear Magnetic Resonance Studies of Cation Transport in Vivo: Na+ Transport in Yeast Cells",
abstract = "A new nuclear magnetic resonance (NMR) method for monitoring transmembrane metal cation transport is reported. It is illustrated with a study of Na+ efflux from Na+-rich yeast cells. The technique involves the use of an anionic paramagnetic shift reagent, present only outside the cells, to induce a splitting of the sodium-23 NMR peak, in this case, into components representing intra- and extracellular Na+. The time course of the efflux is in good agreement with the literature and can be well fitted with a double exponential decay expression. Splitting of the lithium-7 NMR signal from a suspension of Li+-rich respiratory-deficient, petite yeasts is also reported.",
author = "Balschi, {James A.} and Cirillo, {Vincent P.} and Springer, {Charles S.}",
note = "Funding Information: We would like to thank the National Science Foundation (NSF; grant PCM 78-07918 to Dr. Springer and grant PCM 80-5570 to Dr. Cirillo) for support of this work. We would also like to thank Mr. Martin Pike and the staff of the Southern California Regional NMR Facility at Cal Tech (Messrs. Thomas Perkins, Utpal Banerjee, Dr. William R. Croasmun, and Prof. Sunney I. Chan) for their generous aid and hospitality in the use of the Bruker WM-500 (funded by NSF grant CHE 79-16324). The Chan group also kindly allowed the use of their laboratory facilities for the incubation (and explosion) of some of the yeast samples. Our gratitude also goes to Mr. Nelson Ayala and Prof. Albert Haim for the computer fitting in Fig. 2. Received for publication 2 November 1981 and in revised form 8 February 1982.",
year = "1982",
doi = "10.1016/S0006-3495(82)84566-9",
language = "English (US)",
volume = "38",
pages = "323--326",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "3",
}