Direct actions of androgens on the survival, growth and secretion of steroids and anti-Mü llerian hormone by individual macaque follicles during three-dimensional culture

J. K. Rodrigues, P. A. Navarro, Mary Zelinski, Richard Stouffer, Jing Xu

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    studyquestion:What are thedirect effectsof androgensonprimatefollicular development andfunctionat specific stages of folliculogenesis? summaryanswer: Androgen addition altered primate follicle survival, growth, steroid and anti-Müllerian hormone (AMH) production, and oocyte quality in vitro, in a dose-and stage-dependent manner. what is known already: Androgens have local actions in the ovary, particularly in the developing follicles. It is hypothesized that androgen promotes early follicular growth, but becomes detrimental to the antral follicles in primates. study design, size, duration: In vitro follicle maturation was performed using rhesus macaques. Secondary (125-225 μm) follicles were mechanically isolated from 14 pairs of ovaries, encapsulated into alginate (0.25% w/v), and cultured for 40 days. participants/materials, setting, methods: Individual follicles were cultured in a 5% O2 environment, in alpha minimum essential medium supplemented with recombinant human FSH. Follicles were randomly assigned to experiments of steroid ablation by trilostane (TRL), testosterone (T) replacement and dihydrotestosterone (DHT) replacement. Follicle survival and growth were assessed. Follicles with diameters ≥500 μmatWeek 5 were categorized as fast-grow follicles. Pregnenolone (P5), progesterone (P4), estradiol (E2) and AMHconcentrations in media were measured. Meiotic maturation and fertilization of oocytes from recombinant human chorionic gonadotrophin-treated follicles were assessed at the end of culture. main results and the role of chance: Compared with controls, TRL exposure reduced (P

    Original languageEnglish (US)
    Pages (from-to)664-674
    Number of pages11
    JournalHuman Reproduction
    Volume30
    Issue number3
    DOIs
    StatePublished - 2015

    Fingerprint

    Macaca
    Androgens
    Steroids
    Hormones
    Primates
    Oocytes
    Ovary
    Growth
    Human Follicle Stimulating Hormone
    Pregnenolone
    Dihydrotestosterone
    Chorionic Gonadotropin
    Macaca mulatta
    Fertilization
    Progesterone
    Testosterone
    Estradiol
    trilostane
    In Vitro Techniques

    Keywords

    • Androgens
    • Dihydrotestosterone
    • Follicle culture
    • Follicle development
    • Testosterone

    ASJC Scopus subject areas

    • Rehabilitation
    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

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    title = "Direct actions of androgens on the survival, growth and secretion of steroids and anti-M{\"u} llerian hormone by individual macaque follicles during three-dimensional culture",
    abstract = "studyquestion:What are thedirect effectsof androgensonprimatefollicular development andfunctionat specific stages of folliculogenesis? summaryanswer: Androgen addition altered primate follicle survival, growth, steroid and anti-M{\"u}llerian hormone (AMH) production, and oocyte quality in vitro, in a dose-and stage-dependent manner. what is known already: Androgens have local actions in the ovary, particularly in the developing follicles. It is hypothesized that androgen promotes early follicular growth, but becomes detrimental to the antral follicles in primates. study design, size, duration: In vitro follicle maturation was performed using rhesus macaques. Secondary (125-225 μm) follicles were mechanically isolated from 14 pairs of ovaries, encapsulated into alginate (0.25{\%} w/v), and cultured for 40 days. participants/materials, setting, methods: Individual follicles were cultured in a 5{\%} O2 environment, in alpha minimum essential medium supplemented with recombinant human FSH. Follicles were randomly assigned to experiments of steroid ablation by trilostane (TRL), testosterone (T) replacement and dihydrotestosterone (DHT) replacement. Follicle survival and growth were assessed. Follicles with diameters ≥500 μmatWeek 5 were categorized as fast-grow follicles. Pregnenolone (P5), progesterone (P4), estradiol (E2) and AMHconcentrations in media were measured. Meiotic maturation and fertilization of oocytes from recombinant human chorionic gonadotrophin-treated follicles were assessed at the end of culture. main results and the role of chance: Compared with controls, TRL exposure reduced (P",
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    author = "Rodrigues, {J. K.} and Navarro, {P. A.} and Mary Zelinski and Richard Stouffer and Jing Xu",
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    AU - Rodrigues, J. K.

    AU - Navarro, P. A.

    AU - Zelinski, Mary

    AU - Stouffer, Richard

    AU - Xu, Jing

    PY - 2015

    Y1 - 2015

    N2 - studyquestion:What are thedirect effectsof androgensonprimatefollicular development andfunctionat specific stages of folliculogenesis? summaryanswer: Androgen addition altered primate follicle survival, growth, steroid and anti-Müllerian hormone (AMH) production, and oocyte quality in vitro, in a dose-and stage-dependent manner. what is known already: Androgens have local actions in the ovary, particularly in the developing follicles. It is hypothesized that androgen promotes early follicular growth, but becomes detrimental to the antral follicles in primates. study design, size, duration: In vitro follicle maturation was performed using rhesus macaques. Secondary (125-225 μm) follicles were mechanically isolated from 14 pairs of ovaries, encapsulated into alginate (0.25% w/v), and cultured for 40 days. participants/materials, setting, methods: Individual follicles were cultured in a 5% O2 environment, in alpha minimum essential medium supplemented with recombinant human FSH. Follicles were randomly assigned to experiments of steroid ablation by trilostane (TRL), testosterone (T) replacement and dihydrotestosterone (DHT) replacement. Follicle survival and growth were assessed. Follicles with diameters ≥500 μmatWeek 5 were categorized as fast-grow follicles. Pregnenolone (P5), progesterone (P4), estradiol (E2) and AMHconcentrations in media were measured. Meiotic maturation and fertilization of oocytes from recombinant human chorionic gonadotrophin-treated follicles were assessed at the end of culture. main results and the role of chance: Compared with controls, TRL exposure reduced (P

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    KW - Follicle development

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    JF - Human Reproduction

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