Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio)

Phillip M. Uribe, Melissa A. Mueller, Julia S. Gleichman, Matthew D. Kramer, Qi Wang, Martha Sibrian-Vazquez, Robert M. Strongin, Peter Steyger, Douglas A. Cotanche, Jonathan I. Matsui

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Inner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to study drug-induced sensory hair cell death since their hair cells are similar in structure and function to those found in humans. We developed a cisplatin dose-response curve using a transgenic line of zebrafish that expresses membrane-targeted green fluorescent protein under the control of the Brn3c promoter/enhancer. Recently, several small molecule screens have been conducted using zebrafish to identify potential pharmacological agents that could be used to protect sensory hair cells in the presence of ototoxic drugs. Dimethyl sulfoxide (DMSO) is typically used as a solvent for many pharmacological agents in sensory hair cell cytotoxicity assays. Serendipitously, we found that DMSO potentiated the effects of cisplatin and killed more sensory hair cells than treatment with cisplatin alone. Yet, DMSO alone did not kill hair cells. We did not observe the synergistic effects of DMSO with the ototoxic aminoglycoside antibiotic neomycin. Cisplatin treatment with other commonly used organic solvents (i.e. ethanol, methanol, and polyethylene glycol 400) also did not result in increased cell death compared to cisplatin treatment alone. Thus, caution should be exercised when interpreting data generated from small molecule screens since many compounds are dissolved in DMSO.

Original languageEnglish (US)
Article numbere55359
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2013

Fingerprint

cisplatin
Zebrafish
Cell death
dimethyl sulfoxide
Dimethyl Sulfoxide
Danio rerio
Cisplatin
hairs
cell death
Cell Death
Cells
aminoglycoside antibiotics
Aminoglycosides
cells
Pharmacology
Anti-Bacterial Agents
Molecules
Neomycin
drugs
Inner Ear

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Uribe, P. M., Mueller, M. A., Gleichman, J. S., Kramer, M. D., Wang, Q., Sibrian-Vazquez, M., ... Matsui, J. I. (2013). Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio). PLoS One, 8(2), [e55359]. https://doi.org/10.1371/journal.pone.0055359

Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio). / Uribe, Phillip M.; Mueller, Melissa A.; Gleichman, Julia S.; Kramer, Matthew D.; Wang, Qi; Sibrian-Vazquez, Martha; Strongin, Robert M.; Steyger, Peter; Cotanche, Douglas A.; Matsui, Jonathan I.

In: PLoS One, Vol. 8, No. 2, e55359, 01.02.2013.

Research output: Contribution to journalArticle

Uribe, PM, Mueller, MA, Gleichman, JS, Kramer, MD, Wang, Q, Sibrian-Vazquez, M, Strongin, RM, Steyger, P, Cotanche, DA & Matsui, JI 2013, 'Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio)', PLoS One, vol. 8, no. 2, e55359. https://doi.org/10.1371/journal.pone.0055359
Uribe PM, Mueller MA, Gleichman JS, Kramer MD, Wang Q, Sibrian-Vazquez M et al. Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio). PLoS One. 2013 Feb 1;8(2). e55359. https://doi.org/10.1371/journal.pone.0055359
Uribe, Phillip M. ; Mueller, Melissa A. ; Gleichman, Julia S. ; Kramer, Matthew D. ; Wang, Qi ; Sibrian-Vazquez, Martha ; Strongin, Robert M. ; Steyger, Peter ; Cotanche, Douglas A. ; Matsui, Jonathan I. / Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio). In: PLoS One. 2013 ; Vol. 8, No. 2.
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