Diffusion tensor imaging detects early cerebral cortex abnormalities in neuronal architecture induced by bilateral neonatal enucleation: An experimental model in the ferret

Andrew S. Bock, Jaime F. Olavarria, Lindsey A. Leigland, Erin N. Taber, Sune N. Jespersen, Christopher (Chris) Kroenke

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Diffusion tensor imaging (DTI) is a technique that non-invasively provides quantitative measures of water translational diffusion, including fractional anisotropy (FA), that are sensitive to the shape and orientation of cellular elements, such as axons, dendrites and cell somas. For several neurodevelopmental disorders, histopathological investigations have identified abnormalities in the architecture of pyramidal neurons at early stages of cerebral cortex development. To assess the potential capability of DTI to detect neuromorphological abnormalities within the developing cerebral cortex, we compare changes in cortical FA with changes in neuronal architecture and connectivity induced by bilateral enucleation at postnatal day 7 (BEP7) in ferrets. We show here that the visual callosal pattern in BEP7 ferrets is more irregular and occupies a significantly greater cortical area compared to controls at adulthood. To determine whether development of the cerebral cortex is altered in BEP7 ferrets in a manner detectable by DTI, cortical FA was compared in control and BEP7 animals on postnatal day 31. Visual cortex, but not rostrally adjacent non-visual cortex, exhibits higher FA than control animals, consistent with BEP7 animals ossessing axonal and dendritic arbors of reduced complexity than age-matched controls. Subsequent to DTI, Golgi-staining and analysis methods were used to identify regions, restricted to visual areas, in which the orientation distribution of neuronal processes is significantly more concentrated than in control ferrets. Together, these findings suggest that DTI can be of utility for detecting abnormalities associated with neurodevelopmental disorders at early stages of cerebral cortical development, and that the neonatally enucleated ferret is a useful animal model system for systematically assessing the potential of this new diagnostic strategy.

Original languageEnglish (US)
Article number149
JournalFrontiers in Systems Neuroscience
Volume4
DOIs
StatePublished - 2010

Fingerprint

Ferrets
Diffusion Tensor Imaging
Cerebral Cortex
Anisotropy
Theoretical Models
Corpus Callosum
Pyramidal Cells
Carisoprodol
Visual Cortex
Dendrites
Axons
Animal Models
Staining and Labeling
Water

Keywords

  • Brain
  • Diffusion tensor imaging
  • Enucleation
  • Ferret
  • Golgi
  • Interhemispheric callosal connections
  • Magnetic resonance imaging
  • Visual system

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience
  • Cognitive Neuroscience
  • Developmental Neuroscience

Cite this

Diffusion tensor imaging detects early cerebral cortex abnormalities in neuronal architecture induced by bilateral neonatal enucleation : An experimental model in the ferret. / Bock, Andrew S.; Olavarria, Jaime F.; Leigland, Lindsey A.; Taber, Erin N.; Jespersen, Sune N.; Kroenke, Christopher (Chris).

In: Frontiers in Systems Neuroscience, Vol. 4, 149, 2010.

Research output: Contribution to journalArticle

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