TY - JOUR
T1 - Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens
AU - Chou, Sunwen
N1 - Funding Information:
Supported by Department of Veterans Affairs research funds. Reprints and correspondence: Dr. Sunwen Chou, VA Medical Center 1I1I1IFF,, P.O. Box 1034, Portland, OR 997722f(JJ7]..
PY - 1990/9
Y1 - 1990/9
N2 - DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerise, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies.
AB - DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerise, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies.
UR - http://www.scopus.com/inward/record.url?scp=0025029998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025029998&partnerID=8YFLogxK
U2 - 10.1093/infdis/162.3.738
DO - 10.1093/infdis/162.3.738
M3 - Article
C2 - 2167341
AN - SCOPUS:0025029998
SN - 0022-1899
VL - 162
SP - 738
EP - 742
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -