Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens

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Abstract

DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerase, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies.

Original languageEnglish (US)
Pages (from-to)738-742
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number3
StatePublished - Sep 1990

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Cytomegalovirus
DNA Sequence Analysis
Immediate-Early Genes
DNA-Directed DNA Polymerase
Epidemiologic Studies
Glycoproteins
Urine
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

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abstract = "DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerase, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies.",
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