Differentiation of a passive vaccine and the humoral immune response toward infection: Analysis of phage displayed peptides

Dror D. Siman-Tov, Leehee Navon-Perry, Nancy L. Haigwood, Jonathan M. Gershoni

    Research output: Contribution to journalArticle

    4 Scopus citations

    Abstract

    Antibody-genes undergo molecular events that produce unique binding-sites that recognize specific epitopes, thus, leading to B-cell clonal variation. As a result, different binding-site structures (paratope internal images) are produced even when two distinct B-cells bind one and the same epitope. Paratope structural variation can be exploited to enable one to evaluate antibody-diversity in a single polyclonal serum sample. This is accomplished through the selection of antibody-specific peptides isolated from combinatorial phage displayed peptide libraries. As an example, we demonstrate the analysis of macaque sera containing passively administered antibodies, given as a therapeutic vaccine and antibodies actively produced by the virus-infected monkeys.

    Original languageEnglish (US)
    Pages (from-to)607-612
    Number of pages6
    JournalVaccine
    Volume24
    Issue number5
    DOIs
    StatePublished - Jan 30 2006

    Keywords

    • Epitope mapping
    • Passive vaccine
    • Phage display peptide library
    • R. macaque
    • SIV

    ASJC Scopus subject areas

    • Molecular Medicine
    • Immunology and Microbiology(all)
    • veterinary(all)
    • Public Health, Environmental and Occupational Health
    • Infectious Diseases

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