Differentiation of a passive vaccine and the humoral immune response toward infection: Analysis of phage displayed peptides

Dror D. Siman-Tov, Leehee Navon-Perry, Nancy L. Haigwood, Jonathan M. Gershoni

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Antibody-genes undergo molecular events that produce unique binding-sites that recognize specific epitopes, thus, leading to B-cell clonal variation. As a result, different binding-site structures (paratope internal images) are produced even when two distinct B-cells bind one and the same epitope. Paratope structural variation can be exploited to enable one to evaluate antibody-diversity in a single polyclonal serum sample. This is accomplished through the selection of antibody-specific peptides isolated from combinatorial phage displayed peptide libraries. As an example, we demonstrate the analysis of macaque sera containing passively administered antibodies, given as a therapeutic vaccine and antibodies actively produced by the virus-infected monkeys.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalVaccine
Volume24
Issue number5
DOIs
StatePublished - Jan 30 2006
Externally publishedYes

Keywords

  • Epitope mapping
  • Passive vaccine
  • Phage display peptide library
  • R. macaque
  • SIV

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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