Differentiation of a passive vaccine and the humoral immune response toward infection: Analysis of phage displayed peptides

Dror D. Siman-Tov, Leehee Navon-Perry, Nancy Haigwood, Jonathan M. Gershoni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antibody-genes undergo molecular events that produce unique binding-sites that recognize specific epitopes, thus, leading to B-cell clonal variation. As a result, different binding-site structures (paratope internal images) are produced even when two distinct B-cells bind one and the same epitope. Paratope structural variation can be exploited to enable one to evaluate antibody-diversity in a single polyclonal serum sample. This is accomplished through the selection of antibody-specific peptides isolated from combinatorial phage displayed peptide libraries. As an example, we demonstrate the analysis of macaque sera containing passively administered antibodies, given as a therapeutic vaccine and antibodies actively produced by the virus-infected monkeys.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalVaccine
Volume24
Issue number5
DOIs
StatePublished - Jan 30 2006
Externally publishedYes

Fingerprint

Humoral Immunity
humoral immunity
bacteriophages
Bacteriophages
Vaccines
peptides
Antibody Binding Sites
vaccines
Peptides
antibodies
Antibodies
Infection
infection
epitopes
B-lymphocytes
Epitopes
binding sites
B-Lymphocytes
Antibody Diversity
Binding Sites

Keywords

  • Epitope mapping
  • Passive vaccine
  • Phage display peptide library
  • R. macaque
  • SIV

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Differentiation of a passive vaccine and the humoral immune response toward infection : Analysis of phage displayed peptides. / Siman-Tov, Dror D.; Navon-Perry, Leehee; Haigwood, Nancy; Gershoni, Jonathan M.

In: Vaccine, Vol. 24, No. 5, 30.01.2006, p. 607-612.

Research output: Contribution to journalArticle

Siman-Tov, Dror D. ; Navon-Perry, Leehee ; Haigwood, Nancy ; Gershoni, Jonathan M. / Differentiation of a passive vaccine and the humoral immune response toward infection : Analysis of phage displayed peptides. In: Vaccine. 2006 ; Vol. 24, No. 5. pp. 607-612.
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