Differentiating dental pulp cells via RGD-dendrimer conjugates

J. K. Kim, R. Shukla, L. Casagrande, C. Sedgley, J. E. Nör, J. R. Baker, E. E. Hill

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Traumatic dental injuries are often irreversible, underscoring the need for therapies that protect dental pulp cells and enhance their regeneration. We hypothesized that generation 5 poly amido amine (PAMAM) dendrimers (G5), functionalized with fluorescein isothiocyanate (FL) and αVβ3specific, cyclic arginine-glycine-aspartic acid (RGD) peptides, will bind to dental pulp cells (DPCs) and modulate their differentiation. Dental pulp cells and mouse odontoblast-like cells (MDPC-23) (±) treated with G5-FL-RGD were analyzed via Western blot, RT-PCR, and quantitative PCR. Transcription of dental differentiation markers was as follows: Dentin matrix protein (DMP-1), dentin sialoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) as well as vascular endothelial growth factor (VEGF) all increased via the JNK pathway. Long-term G5-RGD treatment of dental pulp cells resulted in enhanced mineralization as examined via Von Kossa assay, suggesting that PAMAM dendrimers conjugated to cyclic RGD peptides can increase the odontogenic potential of these cells.

Original languageEnglish (US)
Pages (from-to)1433-1438
Number of pages6
JournalJournal of dental research
Volume89
Issue number12
DOIs
StatePublished - Dec 1 2010

Keywords

  • PAMAM
  • integrin
  • nanotechnology
  • odontoblasts

ASJC Scopus subject areas

  • Dentistry(all)

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    Kim, J. K., Shukla, R., Casagrande, L., Sedgley, C., Nör, J. E., Baker, J. R., & Hill, E. E. (2010). Differentiating dental pulp cells via RGD-dendrimer conjugates. Journal of dental research, 89(12), 1433-1438. https://doi.org/10.1177/0022034510384870