Differential subcellular localization of SK3-containing channels in the hippocampus

Carmen Ballesteros-Merino, Masahiko Watanabe, Ryuichi Shigemoto, Yugo Fukazawa, John Adelman, Rafael Luján

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Small-conductance, Ca2+-activated K+ (SK) channels are expressed in the hippocampus where they regulate synaptic responses, plasticity, and learning and memory. To investigate the expression of SK3 (KCNN3) subunits, we determined the developmental profile and subcellular distribution of SK3 in the developing mouse hippocampus using western blots, immunohistochemistry and high-resolution immunoelectron microscopy. The results showed that SK3 expression increased during postnatal development, and that the localization of SK3 changed from being mainly associated with the endoplasmic reticulum and intracellular sites during the first postnatal week to being progressively concentrated in dendritic spines during later stages. In the adult, SK3 was localized mainly in postsynaptic compartments, both at extrasynaptic sites and along the postsynaptic density of excitatory synapses. Double labelling showed that SK3 co-localized with SK2 (KCNN2) and with N-methyl-D-aspartate receptors. Finally, quantitative analysis of SK3 density revealed two subcellular distribution patterns in different hippocampal layers, with SK3 being unevenly distributed in CA1 region of the hippocampus pyramidal cells and homogeneously distributed in dentate gyrus granule cells. Our results revealed a complex cell surface distribution of SK3-containing channels and a distinct developmental program that may influence different hippocampal functions.

Original languageEnglish (US)
Pages (from-to)883-892
Number of pages10
JournalEuropean Journal of Neuroscience
Volume39
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Hippocampus
Post-Synaptic Density
Calcium-Activated Potassium Channels
Dendritic Spines
Neuronal Plasticity
Immunoelectron Microscopy
Pyramidal Cells
Dentate Gyrus
N-Methyl-D-Aspartate Receptors
Endoplasmic Reticulum
Synapses
Western Blotting
Immunohistochemistry
Learning

Keywords

  • Cell surface distribution
  • Development
  • Electron microscopy
  • Immunohistochemistry
  • Mouse
  • Potassium channel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ballesteros-Merino, C., Watanabe, M., Shigemoto, R., Fukazawa, Y., Adelman, J., & Luján, R. (2014). Differential subcellular localization of SK3-containing channels in the hippocampus. European Journal of Neuroscience, 39(6), 883-892. https://doi.org/10.1111/ejn.12474

Differential subcellular localization of SK3-containing channels in the hippocampus. / Ballesteros-Merino, Carmen; Watanabe, Masahiko; Shigemoto, Ryuichi; Fukazawa, Yugo; Adelman, John; Luján, Rafael.

In: European Journal of Neuroscience, Vol. 39, No. 6, 2014, p. 883-892.

Research output: Contribution to journalArticle

Ballesteros-Merino, C, Watanabe, M, Shigemoto, R, Fukazawa, Y, Adelman, J & Luján, R 2014, 'Differential subcellular localization of SK3-containing channels in the hippocampus', European Journal of Neuroscience, vol. 39, no. 6, pp. 883-892. https://doi.org/10.1111/ejn.12474
Ballesteros-Merino C, Watanabe M, Shigemoto R, Fukazawa Y, Adelman J, Luján R. Differential subcellular localization of SK3-containing channels in the hippocampus. European Journal of Neuroscience. 2014;39(6):883-892. https://doi.org/10.1111/ejn.12474
Ballesteros-Merino, Carmen ; Watanabe, Masahiko ; Shigemoto, Ryuichi ; Fukazawa, Yugo ; Adelman, John ; Luján, Rafael. / Differential subcellular localization of SK3-containing channels in the hippocampus. In: European Journal of Neuroscience. 2014 ; Vol. 39, No. 6. pp. 883-892.
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