TY - JOUR
T1 - Differential subcellular localization of particular mRNAs in hippocampal neurons in culture
AU - Kleiman, Robin
AU - Banker, Gary
AU - Steward, Oswald
N1 - Funding Information:
We thank Drs. A. Matus, N. Cowan, A. Routtenberg, and M. Kirschner, who provided cDNA clones for MAPZ, NF-68, CAP-43, and J3-tubulin, and to D. Chikaraishi for recloning the neurofilament and tubulin cDNAs into riboprobe vectors. Thanks to Dr. Ted Mifflin and Regina Matulaitis of the Molecular Probe Laboratory of the University of Virginia for producing the labeled cRNA probes used in this study and to Drs. Pat Trimmer and Enrique Torre for their help in developing techniques used in this study. This research was funded by NIH grant NS23094 (to C. B. and 0. 5.); R. K. received support from NIH training grant HD07323.
PY - 1990/12
Y1 - 1990/12
N2 - In situ hybridization was used to assess the subcellular distribution of mRNAs encoding several important neuronal proteins in hippocampal neurons in culture. mRNA encoding GAP-43, a protein that is largely excluded from dendrites, was restricted to nerve cell bodies, as were mRNAs encoding neurofilament-68 and β-tubulin, which are prominent constituents of dendrites and of axons. In contrast, mRNA encoding MAP-2, a protein that is selectively distributed in dendrites and cell bodies, was present in both dendrites and cell bodies. These results demonstrate that different mRNAs are differentially distributed within individual hippocampal neurons. Taken together with previous findings from other laboratories, our results suggest that only a limited set of mRNAs are available for local translation within dendrites.
AB - In situ hybridization was used to assess the subcellular distribution of mRNAs encoding several important neuronal proteins in hippocampal neurons in culture. mRNA encoding GAP-43, a protein that is largely excluded from dendrites, was restricted to nerve cell bodies, as were mRNAs encoding neurofilament-68 and β-tubulin, which are prominent constituents of dendrites and of axons. In contrast, mRNA encoding MAP-2, a protein that is selectively distributed in dendrites and cell bodies, was present in both dendrites and cell bodies. These results demonstrate that different mRNAs are differentially distributed within individual hippocampal neurons. Taken together with previous findings from other laboratories, our results suggest that only a limited set of mRNAs are available for local translation within dendrites.
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U2 - 10.1016/0896-6273(90)90341-C
DO - 10.1016/0896-6273(90)90341-C
M3 - Article
C2 - 2148488
AN - SCOPUS:0025603077
SN - 0896-6273
VL - 5
SP - 821
EP - 830
JO - Neuron
JF - Neuron
IS - 6
ER -