Differential subcellular localization of particular mRNAs in hippocampal neurons in culture

Robin Kleiman, Gary Banker, Oswald Steward

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

In situ hybridization was used to assess the subcellular distribution of mRNAs encoding several important neuronal proteins in hippocampal neurons in culture. mRNA encoding GAP-43, a protein that is largely excluded from dendrites, was restricted to nerve cell bodies, as were mRNAs encoding neurofilament-68 and β-tubulin, which are prominent constituents of dendrites and of axons. In contrast, mRNA encoding MAP-2, a protein that is selectively distributed in dendrites and cell bodies, was present in both dendrites and cell bodies. These results demonstrate that different mRNAs are differentially distributed within individual hippocampal neurons. Taken together with previous findings from other laboratories, our results suggest that only a limited set of mRNAs are available for local translation within dendrites.

Original languageEnglish (US)
Pages (from-to)821-830
Number of pages10
JournalNeuron
Volume5
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

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Dendrites
Neurons
Messenger RNA
GAP-43 Protein
Intermediate Filaments
Tubulin
In Situ Hybridization
Axons
Proteins
Cell Body

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential subcellular localization of particular mRNAs in hippocampal neurons in culture. / Kleiman, Robin; Banker, Gary; Steward, Oswald.

In: Neuron, Vol. 5, No. 6, 1990, p. 821-830.

Research output: Contribution to journalArticle

Kleiman, Robin ; Banker, Gary ; Steward, Oswald. / Differential subcellular localization of particular mRNAs in hippocampal neurons in culture. In: Neuron. 1990 ; Vol. 5, No. 6. pp. 821-830.
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