TY - JOUR
T1 - Differential staining of human and murine chromatin in situ by hybridization with species-specific satellite DNA probes
AU - Weier, Heinz Ulrich G.
AU - Zitzelsberger, Horst F.
AU - Gray, Joe W.
N1 - Funding Information:
This work was supported in part by NICHD grant HD17665 and NC1 grant CA4.5919. Additional support was provided by the Program for Analytical Cytology.
PY - 1992/2/14
Y1 - 1992/2/14
N2 - Human and murine chromatin was differentially labeled by hybridization with DNA probes that bind to species-specific satellite DNA. The targets for in situ hybridization were the mouse-specific major or gamma satellite DNA and the human alpha satellite DNA. These sequences typically are localized at or near the chromosome centromeres, and remain their tight localization throughout the cell cycle. DNA probes were synthesized in vitro by primer directed DNA amplification using the polymerase chain reaction. In typical applications like the differentiation of cells derived from chimeric animals or the characterization of chromosomes in somatic cell hybrids, the two DNA probes are differently labeled and detected using label-specific reagents that fluoresce at different wavelengths. The rapid technique for chromatin discrimination described here combines high specificity with unprecedented signal intensity.
AB - Human and murine chromatin was differentially labeled by hybridization with DNA probes that bind to species-specific satellite DNA. The targets for in situ hybridization were the mouse-specific major or gamma satellite DNA and the human alpha satellite DNA. These sequences typically are localized at or near the chromosome centromeres, and remain their tight localization throughout the cell cycle. DNA probes were synthesized in vitro by primer directed DNA amplification using the polymerase chain reaction. In typical applications like the differentiation of cells derived from chimeric animals or the characterization of chromosomes in somatic cell hybrids, the two DNA probes are differently labeled and detected using label-specific reagents that fluoresce at different wavelengths. The rapid technique for chromatin discrimination described here combines high specificity with unprecedented signal intensity.
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U2 - 10.1016/0006-291X(92)91876-R
DO - 10.1016/0006-291X(92)91876-R
M3 - Article
C2 - 1540174
AN - SCOPUS:0026523718
SN - 0006-291X
VL - 182
SP - 1313
EP - 1319
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -