Differential staining of human and murine chromatin in situ by hybridization with species-specific satellite DNA probes

Heinz Ulrich G Weier, Horst F. Zitzelsberger, Joe Gray

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Human and murine chromatin was differentially labeled by hybridization with DNA probes that bind to species-specific satellite DNA. The targets for in situ hybridization were the mouse-specific major or gamma satellite DNA and the human alpha satellite DNA. These sequences typically are localized at or near the chromosome centromeres, and remain their tight localization throughout the cell cycle. DNA probes were synthesized in vitro by primer directed DNA amplification using the polymerase chain reaction. In typical applications like the differentiation of cells derived from chimeric animals or the characterization of chromosomes in somatic cell hybrids, the two DNA probes are differently labeled and detected using label-specific reagents that fluoresce at different wavelengths. The rapid technique for chromatin discrimination described here combines high specificity with unprecedented signal intensity.

Original languageEnglish (US)
Pages (from-to)1313-1319
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
StatePublished - Feb 14 1992
Externally publishedYes

Fingerprint

Satellite DNA
DNA Probes
Chromatin
In Situ Hybridization
Chromosomes
Staining and Labeling
Cells
DNA Primers
Centromere
Hybrid Cells
Polymerase chain reaction
Amplification
Labels
Cell Differentiation
Cell Cycle
Animals
Wavelength
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Differential staining of human and murine chromatin in situ by hybridization with species-specific satellite DNA probes. / Weier, Heinz Ulrich G; Zitzelsberger, Horst F.; Gray, Joe.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 3, 14.02.1992, p. 1313-1319.

Research output: Contribution to journalArticle

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